【作者】 張慧; 江秀紅; 林新堅(jiān); 陳濟(jì)?。?林戎斌; 鄭永標(biāo);
【Author】 ZHANG Hui1,JIANG Xiuhong2,LIN Xinjian1,CHEN Jichen1,LIN Rongbin1,ZHENG Yongbiao1 1The Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;2Fujian Provincial Quality Safety Inspection and Test Center for Agricultural Products,Fuzhou,Fujian 350003,China
【機(jī)構(gòu)】 福建省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 福建省農(nóng)產(chǎn)品質(zhì)量安全檢驗(yàn)檢測中心;
【摘要】 利用同工酶I、SSR分子標(biāo)記技術(shù)等方法對3個(gè)離子束選育獲得的姬松茸菌株(AbML2,AbML7和AbML11)和一株孢子分離株(AbMD3)與出發(fā)菌株進(jìn)行鑒定。結(jié)果表明:AbML11、AbML7在酯酶同工酶、多酚氧化酶譜帶數(shù)量上與AbM9有較大的差別,有特征帶出現(xiàn);ISSR聚類分析看出,在相似系數(shù)大約為0.64水平上,可將供試菌株分為兩大類,AbML2、AbMD3、AbML7、AbM9歸為一類,菌株AbML11單獨(dú)聚為一類。
【Abstract】 Three Agaricus blazei mutants(AbML2,AbML7 and AbML11) generated by nitrogen ion beam injection,and one spore isolate(AbMD3),all derived from parental strain AbML11,were compared using isozyme and ISSR markers.Esterase and polyphenol oxidase isozyme zymograms of AbML11,AbML7 and AbM9 were distinct.Analysis of ISSR markers separated the isolates into two groups at a genetic distance of 0.64,one consisting of AbML2、AbMD3、AbML7 and AbM9,and the other of AbML11.
【關(guān)鍵詞】 食用菌; 同工酶; PCR技術(shù);
【Key words】 Edible fungi; esterase isozyme; PCR technique;
【基金】 福建省重點(diǎn)科技項(xiàng)目(編號:2008Y0024,2008Y0025,2009Y1005);;福建省科技廳項(xiàng)目(編號:2002I010);;福建省財(cái)政專項(xiàng)(編號:STIF-Y01)的部分研究內(nèi)容
【分類號】S646.15