【作者】 張文雋; 吳亞召; 雷萍; 孫悅迎;
【Author】 ZHANG Wen-jun,WU Ya-zhao,LEI Ping,SUN Yue-ying(Shanxi Microbioogy Research Institute,Xi’an Shanxi 710043)
【機(jī)構(gòu)】 陜西省微生物研究所;
【摘要】 研究了樺樹桑黃液體發(fā)酵培養(yǎng)基中不同營(yíng)養(yǎng)成分對(duì)樺樹桑黃菌絲生長(zhǎng)的影響。采用L9(34)正交設(shè)計(jì)法對(duì)樺樹桑黃發(fā)酵培養(yǎng)基進(jìn)行了優(yōu)化,初步篩選出了樺樹桑黃液體發(fā)酵的培養(yǎng)基。結(jié)果表明,優(yōu)化培養(yǎng)基為玉米粉1%、麥麩1%、葡萄糖2%、蛋白胨0.5%、酵母膏0.5%、磷酸二氫鉀0.1%、硫酸鎂0.05%、維生素B1 1 mg.100-1.mL-1.
【Abstract】 The effects of cultural compounds on the growth of Phellinus igniarius growing on silver birch tree were investigated.The optimum submerged fermentation parameters for Phellinus igniarius growing on silver birch tree were studied by orthogonal test.The results showed that the optimal cuiture medium were corn 1%,bran 1%,glucose 2%,peptone 0.5%,yeast extract 0.5%,KH2PO4 0.1%,MgSO4 0.05% and VB1 1 mg.100-1·mL-1.
【關(guān)鍵詞】 樺樹桑黃; 液體培養(yǎng); 碳源; 氮源; 最適培養(yǎng)基;
【Key words】 Phellinus igniarius growing on birch tree; Liquid culture; Carbon source; Nitrogen source; Optimal medium;
【基金】 陜西省科學(xué)院項(xiàng)目“桑黃菌液體發(fā)酵及其桑黃多糖提取技術(shù)研究”,項(xiàng)目編號(hào):2009K-10;;西安市科技局工業(yè)應(yīng)用技術(shù)研發(fā)項(xiàng)目“藥用真菌桑黃液體發(fā)酵技術(shù)研究”,項(xiàng)目編號(hào):CXY09023(6)
【文獻(xiàn)出處】 中國(guó)食用菌, Edible Fungi of China, 編輯部郵箱 , 2010年06期
【DOI】CNKI:SUN:ZSYJ.0.2010-06-011