劉開輝; 鄧百萬; 陳文強(qiáng); 丁小維; 彭浩; 解修超
陜西理工學(xué)院生物科學(xué)與工程學(xué)院陜西省食藥用菌工程技術(shù)研究中心
【中文摘要】 對不同形態(tài)豬苓(Polyporus umbellata)菌核(豬屎苓、鐵蛋豬屎苓和雞屎苓)的rDNA(18S r DNA,ITS1-5.8S rDNA-ITS2)和β-tub1進(jìn)行擴(kuò)增、測序。序列分析表明:所得rDNA和β-tub1序列完全相同。以Neighbor-joining(N-J)方法構(gòu)建ITS(ITS1-5.8S rDNA-ITS2)序列系統(tǒng)發(fā)育樹,供試菌株同GenBank中收錄豬苓(P.umbellata)類聚于同一分枝且序列完全相同,表明不同形態(tài)菌核的豬苓之間親緣關(guān)系較近。
【英文摘要】 Genetic relationships between three Polyporus umbellata strains,designated Zhushiling,Tiedanzhushiling and Jishiling on the basis of differences in sclerotium morphology,were analysed using rDNA(18S rDNA,ITS1-5.8S rDNA-ITS2) and β-tub1 fragment sequences.Although the test strains exhibited dissimilar sclerotial features,no differences were detected in their rDNA and β-tub1 sequences.Using the neighbor-joining method,a phylogenetic tree was constructed on the basis of the ITS data and other ITS sequences deposited in GenBank.This revealed a relatively high genetic similarity between the test strains,another P.umbellata strain and several Grifola umbellata strains.
【中文關(guān)鍵詞】 豬苓; 菌核; rDNA序列; β-tub1序列; 親源關(guān)系
【英文關(guān)鍵詞】 Polyporus umbellata; sclerotium; rDNA sequence; β-tub1 sequence; genetic relationship
【基金】陜西省教育廳項(xiàng)目(編號:04JS32,08JZ21,08JK244,08JK248);; 陜西理工學(xué)院人才引進(jìn)項(xiàng)目(編號:SLGQDO712,SLGQDO713)的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-003