王守現(xiàn); 劉宇; 張英春; 耿小麗; 許峰; 趙爽; 孟莉莉
北京市農(nóng)林科學(xué)院植保環(huán)保研究所

【中文摘要】 應(yīng)用RAPD技術(shù)對(duì)13個(gè)蜜環(huán)菌(Ar millaria mellea)菌株進(jìn)行了遺傳多樣性分析。結(jié)果表明,在供試的60條RAPD隨機(jī)引物中,有16條可擴(kuò)增出清晰、穩(wěn)定的DNA條帶;聚類分析表明,以黃綠蜜環(huán)菌(Ar millaria luteo-virens)為外類群,在相似系數(shù)0.760水平時(shí),可將供試的13個(gè)菌株分成四大類:第一大類為A.m0010、洋縣M-8、蜜0903、A.m0005、寧強(qiáng)A9-1、蜜環(huán)菌A9和A.m0006,第二大類為A.m0007、A.m0004和A.m0008;第三大類為蜜金鄉(xiāng)和蜜三明;第四大類為A.m0001。研究結(jié)果表明RAPD技術(shù)可以有效區(qū)分蜜環(huán)菌菌株并分析其遺傳多樣性。

【英文摘要】 Genetic diversity among thirteen Armillaria mellea strains was analyzed by RAPD using sixteen primers selected from 60 tested primers that displayed specific and stable amplification patterns.A dendrogram constructed using the amplification data and Armillaria luteo-virens as the out-group separated the test strains into four groups at a coefficient of 0.760.Group I consisted of strains A.m0010,Yangxian M-8,Mi 0903,A.m0005,Ningqiang A9-1,Mihuanjun A9 and A.m 0006,Group II of strains A.m0007,A.m0004 and A.m0008,Group III of strains Mi Jinxiang and Mi Sanming,and Group IV of strain A.m0001.Our data showed that RAPD was a suitable method for differentiating the test strains and was effective in reflecting genetic diversity.

【中文關(guān)鍵詞】 蜜環(huán)菌; RAPD分析; 遺傳多樣性
【英文關(guān)鍵詞】 Armillaria mellea; RAPD analysis; genetic diversity
【基金】北京市農(nóng)林科學(xué)院青年基金項(xiàng)目(編號(hào):2007010810)的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-004