朱惠照; 李明焱; 楚桐麗; 魏美芝; 鄭化先; 劉楠
浙江壽仙谷生物科技有限公司; 金華壽仙谷藥業(yè)有限公司
【中文摘要】 通過(guò)對(duì)選育出的赤芝(Ganoderma lucidum)品種—"仙芝1號(hào)"采用拮抗試驗(yàn)、分子生物學(xué)實(shí)驗(yàn)進(jìn)行遺傳分析,結(jié)果表明"仙芝1號(hào)"同"仙源5號(hào)"及韓芝有明顯拮抗效應(yīng),其ERIC-PCR擴(kuò)增圖譜與其它品種差異明顯,因此選育出的"仙芝1號(hào)"是不同于其他靈芝品種的赤芝新品種。
【英文摘要】 Antagonism tests and PCR amplification methodology were used to identify a strain of Ganoderma lucidum designated Xianzhi 1.The original parental strain was isolated from a fruit body collected from the wild and was subjected to subsequent long-term selection procedures.Xianzhi 1 exhibited an antagonistic reaction with a selected intermediate parent strain,Xianyuan 5,and several other G.lucidum cultivars.PCR amplification revealed a band that was specific for G.lucidum,and ERIC-PCR generated an amplification pattern that distinguished Xianzhi 1 from ten other G.lucidum cultivars indicating that it was a new strain.
【中文關(guān)鍵詞】 “仙芝1號(hào)”赤芝; 拮抗試驗(yàn); ERIC-PCR
【英文關(guān)鍵詞】 Ganoderma lucidum; strain Xianzhi 1; antagonistic reaction; ERIC-PCR
【基金】浙江省重點(diǎn)農(nóng)業(yè)科研項(xiàng)目“精深加工用靈芝品種選育及高效配套栽培技術(shù)研究”(編號(hào):2006C22079)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-006