黃龍花; 吳清平; 楊小兵; 張菊梅
中國(guó)科學(xué)院南海海洋研究所; 廣東省微生物研究所; 廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 中國(guó)科學(xué)院研究生院
【中文摘要】 針對(duì)我國(guó)栽培鳳尾菇(Pleurotus sajor-caju)及其近緣種共10個(gè)菌株的ITS序列,應(yīng)用特異引物進(jìn)行PCR擴(kuò)增,對(duì)其產(chǎn)物進(jìn)行測(cè)序,并從GenBank上下載側(cè)耳屬(Pleurotus)3個(gè)種7條ITS序列、韌傘屬(Lentinus)的4條ITS序列和香菇屬(Lentinula)的3條ITS序列,以冬蟲夏草(Cordyceps sinensis)和蛹蟲草(Cordyceps militaris)為外類群,用NJ(Neighbor-joining)法構(gòu)建系統(tǒng)發(fā)育樹,結(jié)果表明:鳳尾菇在側(cè)耳屬分支上與側(cè)耳屬內(nèi)種的相似性達(dá)98.5%以上,韌傘屬和香菇屬的屬內(nèi)相似性也均為98%以上,而側(cè)耳屬和韌傘屬、香菇屬的屬間相似性僅約80%,故鳳尾菇應(yīng)歸于側(cè)耳屬;在進(jìn)一步研究中,將該鳳尾菇與其近緣種的ITS1-5.8S-ITS2序列作堿基完全比對(duì),結(jié)果發(fā)現(xiàn)它與肺形側(cè)耳(Pleurotus pulmonarius)的堿基完全一致,而與其它近緣種均存在較大堿基差異,因此,目前我國(guó)廣泛栽培的鳳尾菇與肺形側(cè)耳可能是同物異名種,建議訂正其名稱,將其拉丁名統(tǒng)一為P.pulmonarius。本研究首次將ITS序...更多列分析和堿基比對(duì)結(jié)合對(duì)鳳尾菇的分類地位及拉丁名進(jìn)行研究,結(jié)果表明ITS序列分析在近緣種的分類鑒定上很有價(jià)值。 還原
【英文摘要】 In order to clarify the taxonomic status of Pleurotus sajor-caju,we determined ITS sequences from six Pleurotus (including one strain of P. sajor-caju) and four Lentinula strains cultivated in China. Data were combined with ITS sequences from 11 related strains,including Pleurotus,Lentinus,Lentinula,Cordyceps and Ophiocordyceps,obtained from GenBank to construct a phylogenetic tree using the Neighbor-joining method with Cordyceps militaris and Ophiocordyceps sinensis as the outgroups. The P. sajor-caju test strain was located on a branch that incorporated all the other Pleurotus strains examined with a shared similarity value >98.5%. Similarity values among Lentinus and Lentinula strains was also >98%,but only 80% when all three genera were compared. In a further "complete alignment"study,comparison of sections of ITS1-5.8S-ITS2 sequences from four P. sajor-caju strains with corresponding sequences from strains of P. pulmonarius,P.ostreatus and P. eryngii revealed that the sequen...更多ces from P. sajor-caju and P. pulmonarius strains were identical but were different from the other Pleurotus strains. Our data indicate that the P. sajor-caju test strain was synonymous with P. pulmonarius and recommend that it be renamed accordingly. 還原
【中文關(guān)鍵詞】 鳳尾菇; 肺形側(cè)耳; ITS序列; 系統(tǒng)發(fā)育樹
【英文關(guān)鍵詞】 Pleurotus sajor-caju; Pleurotus pulmonarius; ITS sequence; phylogenetic tree
【基金】廣東省科技計(jì)劃項(xiàng)目(編號(hào):2008B050300001,2008A020100024)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-006