李河; 周國英; 劉君昂
中南林業(yè)科技大學(xué)生物技術(shù)開放性中心實驗室; 中南林業(yè)科技大學(xué)林學(xué)院

【中文摘要】 對引起雙孢蘑菇(Agaricus bisporus)褐腐病的病原菌進行分離和分子鑒定。分離培養(yǎng)褐腐病病原菌,采用CTAB法抽提其基因組總DNA,利用真菌通用引物ITS1和ITS4擴增菌株rDNA ITS區(qū)序列,擴增產(chǎn)物純化后克隆轉(zhuǎn)化至大腸桿菌,挑選克隆成功的菌株進行測序。測序結(jié)果在GenBank中進行同源性搜索,并下載部分具有代表性種的ITS序列,利用軟件MEGA4構(gòu)建分子系統(tǒng)發(fā)育樹,通過序列分析,鑒定出引起雙孢蘑菇褐腐病的病原菌為Mycogoneperniciosa。

【英文摘要】 Strain CSUFT0821,a pathogen causing brown rot of Agaricus bisporus,has been identified by determining the sequence of a ribosomal DNA internal transcribed spacer (ITS) and comparing with corresponding sequences deposited in GenBank. Homology was highest (99%) with an ITS sequence from Mycogone perniciosa (EU380317). A phylogenetic tree constructed on the basis of ITS sequences revealed very close proximity between EU380317 and the ITS sequence from strain CSUFT0821,and confirmed that the latter was M. perniciosa.

【中文關(guān)鍵詞】 雙孢蘑菇; 褐腐病; 轉(zhuǎn)錄間隔區(qū); 分子鑒定
【英文關(guān)鍵詞】 Agaricus bisporus; brown rot; internal transcribed spacers (ITS); molecular identification
【基金】國家林業(yè)局“948”項目(編號:2004-4-28)的部分研究內(nèi)容

【文獻出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-015