宋春艷; 尚曉冬; 譚琦; 劉德云; 鄭巧平; 項壽南
中華人民共和國農(nóng)業(yè)部應用真菌資源與利用重點開放實驗室上海市食用菌工程技術研究中心上海市農(nóng)業(yè)遺傳育種重點實驗室上海市農(nóng)業(yè)科學院食用菌研究所; 浙江省麗水市農(nóng)業(yè)科學研究所

【中文摘要】 以香菇菌種939和本所實驗菌株中19為親本,進行原生質體單核體分離和配對雜交。對雜交組合和親本菌株的菌蓋直徑、厚度,菌柄長度、直徑等性狀進行考察。結果表明,10個雜交組合在子實體外觀形態(tài)上與親本存在明顯的差異,且原生質體單核體雜交后代的農(nóng)藝性狀表現(xiàn)規(guī)律與親本交配型的類型存在一定的關聯(lián)。

【英文摘要】 Protoplast monokaryons were prepared from Lentinula edodes,strains 939 and Zhong-19,and eight and two protoplast monokaryons respectively from the two parent strains were intercrossed after determining the mating type.Ten hybrid offspring strains were selected based on mycelial growth rate and used in fruiting tests.Comparison of fruit body characteristics of both hybrid and parental strains revealed variations in the diameter and thickness of fruit body pilei,and the length and diameter of fruit body stipes,which were related to the mating type.

【中文關鍵詞】 原生質體單核體; 雜交育種; 交配型基因; 性狀變異
【英文關鍵詞】 Protoplast monokaryon; crossbreeding; mating type gene; character variation
【基金】上海市科技興農(nóng)重點攻關項目[編號:滬農(nóng)科攻字(2004)第2-16號]的部分研究內(nèi)容

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-001