賈定洪; 鄭林用; 王波; 郭勇; 甘炳成; 黃忠乾; 彭衛(wèi)紅
四川省農(nóng)業(yè)科學(xué)院土壤肥料研究所

【中文摘要】 以7個(gè)栽培菌株為參照,采用ISSR分子標(biāo)記技術(shù)對(duì)5個(gè)野生木耳屬菌株進(jìn)行分析,使用NTSYSpc2.1生物軟件對(duì)12個(gè)供試菌株進(jìn)行聚類分析,構(gòu)建系統(tǒng)樹。試驗(yàn)篩選出了11個(gè)擴(kuò)增譜帶清晰、多態(tài)性好的ISSR引物,共擴(kuò)增出78條清晰易辨的多態(tài)性譜帶。聚類分析結(jié)果表明,利用ISSR技術(shù)可將全部供試材料區(qū)分開,遺傳相似系數(shù)變異范圍為0.1080.822,在相似系數(shù)為0.51時(shí),12個(gè)菌株聚為6個(gè)群,其中5個(gè)野生菌株各自聚為不同類群,遺傳差異較大。

【英文摘要】 Five wild-type Auricularia strains were identified by ISSR(inter-simple sequence repeat)methodology using seven cultivated Auricularia strains as controls.Eleven ISSR primers,selected from a total of 60 test ISSR primers,amplified 78 distinct polymorphic DNA bands from the 12 Auricularia strains.ISSR similarity coefficients ranged from 0.108 to 0.822,and a dendrogram based on UPGMA(unweighted pair group method with arithmetic average) cluster analysis using these values revealed that the 12 strains were clustered into six groups(similarity coefficient 0.51).The five wild-type strains clustered into five different groups,and the most distinct strain genetically was the wild-type strain,Ausm(similarity coefficient 0.198).

【中文關(guān)鍵詞】 木耳屬; ISSR; 遺傳鑒定
【英文關(guān)鍵詞】 Auricularia; inter-simple sequence repeats; genetic identification
【基金】四川省財(cái)政育種工程資助項(xiàng)目“食用菌新品種選育及新材料新方法研究”(編號(hào):2006YZCC09-024);; 四川省育種攻關(guān)資助項(xiàng)目“優(yōu)質(zhì)豐產(chǎn)黑木耳新品種選育”(編號(hào):YZGG06-04-01)的部分研究內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-002