孫巍; 夏春雨; 趙祥杰; 陳智毅; 鄒宇曉; 施英; 劉學(xué)銘
廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實驗室

【中文摘要】 優(yōu)化了以桑葉和麩皮為主要原料由毛云芝菌(Coriolus hirsutus)生產(chǎn)漆酶的培養(yǎng)條件,即培養(yǎng)料起始pH、裝料量、封口情況、接種量、發(fā)酵時間對漆酶活性的影響,并觀察了該菌在優(yōu)化后培養(yǎng)條件下的代謝動力學(xué)情況。結(jié)果表明,自然pH,裝量為10 g/250 mL三角瓶、封口紗布4層,接種量為5 mL種子液/瓶,培養(yǎng)時間為10 d,酶活達到1.37×104U,優(yōu)化后結(jié)果比初始設(shè)計提高了18%。

【英文摘要】 Substrate composition,initial pH,ratio of substrate to cultivation vessel volume,aeration,inoculum size and cultivation time were optimized for maximal laccase production by Coriolus hirsutus grown under solid-state conditions using mulberry leaves and wheat bran as the main components of the cultivation substrate.Highest laccase levels(1.37 × 104 U per) were recorded after ten days in 250 mL culture flasks containing 10 g substrate at pH 6,sealed with four layers of cheese cloth and inoculated with 5 mL of seed culture.

【中文關(guān)鍵詞】 Coriolus hirsutus; 漆酶; 培養(yǎng)條件
【英文關(guān)鍵詞】 Coriolus hirsutus; laccase; optimized cultivation conditions
【基金】教育部留學(xué)回國科研啟動基金(教外司留[2007]1108號);; 廣東省科技攻關(guān)項目(編號:2006B13001004)的部分研究內(nèi)容

【文獻出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-017