沈霞; 余勝光
嘉應(yīng)學(xué)院梅州師范分院

【中文摘要】 研究了稀土元素鑭(La)對(duì)灰樹(shù)花菌絲體富集有機(jī)硒的影響。結(jié)果表明,鑭對(duì)灰樹(shù)花菌絲富硒有促進(jìn)作用,當(dāng)玉米培養(yǎng)基中鑭添加濃度為100 mg/L時(shí),菌絲產(chǎn)量和有機(jī)硒轉(zhuǎn)化率最高。借用正交試驗(yàn)表設(shè)計(jì)組合試驗(yàn),結(jié)果表明,采用馬鈴薯培養(yǎng)基,培養(yǎng)時(shí)間8 d,鑭和硒的添加濃度分別為100 mg/L和50 mg/L,在此條件下,菌絲產(chǎn)量19.52 g/L,硒轉(zhuǎn)化率達(dá)16.48%。

【英文摘要】 The effect of lanthanum supplementation on selenium accumulation by Grifola frondosa mycelium grown in submerged culture was evaluated.When grown in a corn powder-based medium supplemented with different concentrations of lanthanum(20-300 mg/L),the maximum mycelial yield achieved was 18.58 mg/L,and 15.72% of the selenium initially present in the culture medium(50 mg/L) accumulated in the fungal mycelium.Combination tests involving fungal growth in four types of basal media(wheat bran,soybean,potato,corn powder) supplemented with different concentrations of selenium and lanthanum,and for different culture periods,revealed that the optimal conditions for selenium accumulation were eight days growth in a potato-based medium supplemented with 100 mg/L La and 50 mg/L Se.Under these conditions,the mycelial growth yield was 19.52 g/L,and 16.48% of the added Se accumulated in the fungal biomass.

【中文關(guān)鍵詞】 灰樹(shù)花; 液體培養(yǎng); 鑭; 硒
【英文關(guān)鍵詞】 Grifola frondosa; submerged culture; lanthanum supplementation; selenium accumulation
【基金】梅州市科技計(jì)劃項(xiàng)目“灰樹(shù)花深層發(fā)酵培養(yǎng)基配方的優(yōu)化研究”(編號(hào):2006B30)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-020