張慧麗; 楊松; 寇巍; 辛茹; 李玉; 劉宏生
遼寧大學(xué)生命科學(xué)院; 沈陽(yáng)軍區(qū)藥品檢驗(yàn)所; 遼寧大學(xué)生命科學(xué)院; 遼寧大學(xué)生命科學(xué)院; 遼寧大學(xué)生命科學(xué)院; 遼寧大學(xué)生命科學(xué)院 沈陽(yáng)110036; 沈陽(yáng)110026; 沈陽(yáng)110036; 沈陽(yáng)110036; 沈陽(yáng)110036; 沈陽(yáng)110036

【中文摘要】 采用正交試驗(yàn)優(yōu)化樺褐孔菌子實(shí)體多糖的超聲提取工藝條件,并對(duì)提取多糖的抗腫瘤活性進(jìn)行研究。結(jié)果表明,樺褐孔菌多糖超聲提取的最佳工藝條件為超聲時(shí)間70min,溫度80℃,頻率20kHz,加水量12倍水,其多糖在體外對(duì)肝癌HEPG2細(xì)胞株具有明顯的抑制作用,并可誘導(dǎo)腫瘤細(xì)胞凋亡,體內(nèi)實(shí)驗(yàn)發(fā)現(xiàn)多糖可顯著抑制小鼠S180肉瘤的生長(zhǎng),并具有增強(qiáng)免疫作用。

【英文摘要】 Optimal conditions for extraction of polysaccharide from Inonotus obliquus fruit bodies(IOP)were determined as follows:extraction time,70 min;temperature,80 ℃;ratio of aqueous solvent to fruit body,12∶1;and ultrasound frequency,20 kHz.IOP at a concentration of 1.44 μg/mL inhibited cancer cell growth by65% as measured using a tetrazolium-based(MTT)assay to evaluate the anti-proliferative effect of the polysaccharide on hepatocarcinoma HEPG2 cells.A 27% and 35% reduction in tumor weight was also observed in S-180 tumor-bearing mice administered low(50 mL/kg·d,8 μg/mL)and high(100 mL/kg·d,16 μg/mL)doses of IOP,respectively.

【中文關(guān)鍵詞】 樺褐孔菌子實(shí)體多糖; 正交實(shí)驗(yàn); 超聲提取; 肝癌細(xì)胞; 吞噬指數(shù)K
【英文關(guān)鍵詞】 Inonotus obliquus; Polysaccharide; Ultrasound extraction; Cancer cell line

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2007年04期 【DOI】CNKI:SUN:SYJB.0.2007-04-013