羅聯(lián)忠; 林樹(shù)錢(qián); 謝寶貴; 林志彬
福州綠谷生物藥業(yè)技術(shù)研究所; 福州綠谷生物藥業(yè)技術(shù)研究所; 福建農(nóng)林大學(xué)菌物研究中心; 福州綠谷生物藥業(yè)技術(shù)研究所 福州350002; 福建農(nóng)林大學(xué)菌物研究中心; 福州350002 ; 福州350002 ; 福州350002 ; 福州350002; 北京大學(xué)醫(yī)學(xué)部藥理系; 北京100083
【中文摘要】 CTAB法提取靈芝菌絲體總DNA,再以核糖體DNA轉(zhuǎn)錄間隔區(qū)(ITSⅡ、IGRⅠ-PCR RFLP)結(jié)合隨機(jī)引物擴(kuò)增多態(tài)性(RAPD)標(biāo)記分析30個(gè)靈芝菌株間的親緣關(guān)系。結(jié)果表明,ITSⅡI、GRⅠ-PCR RFLP產(chǎn)生了47條帶,其中42條顯示出多態(tài)性,通過(guò)聚類(lèi)分析將30個(gè)菌株分成七大類(lèi),包括紫芝、黑靈芝、樹(shù)舌靈芝、薄樹(shù)靈芝四大類(lèi),以及另外三個(gè)無(wú)法確定具體歸屬的類(lèi)群,但沒(méi)能將赤芝與松杉靈芝區(qū)分開(kāi)。RAPD從247個(gè)隨機(jī)引物中篩選出8個(gè)隨機(jī)引物,共檢測(cè)到69個(gè)多態(tài)性位點(diǎn)并全部顯示出多態(tài)性,通過(guò)聚類(lèi)分析將供試的30個(gè)靈芝菌株全部區(qū)分開(kāi)。
【英文摘要】 Total DNA of Ganoderma mycelium was extracted by the method of CTAB,and genetic relationships among thirty Ganoderma strains were analyzed based on ITS Ⅱ、IGR Ⅰ-PCR RFLP and RAPD.The results showedthat 47 bands were obtained based on ITSⅡ,IGR Ⅰ-PCR RFLP,and 42 bands of them were polymorphic.Thirty strains were divided into seven species: Ganoderma sinense,Ganoderma atrum,Ganoderma applanatum,Ganaderma capense(Loyd) Teng and other three groups(their species are still unknown) through dendrogram aralysis.Only 8 primers produced stable results among 247 random primers by RAPD.Total 69 loci were obtained by using 8 primers and all of them were polymorphic. Based on these 69 loci,thirty Ganoderma strains were differentiated.
【中文關(guān)鍵詞】 靈芝; 核糖體DNA轉(zhuǎn)錄間隔區(qū); 隨機(jī)引物擴(kuò)增多態(tài)性
【英文關(guān)鍵詞】 Ganoderma; Internal transcribed spacer(ITS); Rondom amplified polymorphic DNA(RAPD)
【基金】綠谷集團(tuán)有限公司靈芝專(zhuān)項(xiàng)科研資金全額資助項(xiàng)目“靈芝菌種DNA鑒別”(項(xiàng)目編號(hào):2003SHGV01);; 福建省星火計(jì)劃項(xiàng)目“福建食用菌集聚產(chǎn)業(yè)科技服務(wù)體系建設(shè)示范——食用菌種質(zhì)資源庫(kù)建設(shè)、分類(lèi)與認(rèn)定前期技術(shù)研究”(項(xiàng)目編號(hào):2003EAT20021)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2005年03期 【DOI】CNKI:SUN:SYJB.0.2005-03-001