周昌艷; 唐慶九; 賈薇; 楊焱; 張勁松; 樊華
上海農(nóng)業(yè)科學(xué)院食用菌研究所; 上海農(nóng)業(yè)科學(xué)院食用菌研究所; 上海農(nóng)業(yè)科學(xué)院食用菌研究所; 上海農(nóng)業(yè)科學(xué)院食用菌研究所; 上海農(nóng)業(yè)科學(xué)院食用菌研究所; Institut fur Molekularbiologie und Biochemie; Freie Universitt; Berlin; Germany 上海201106; Institut fur Molekularbiologie und Biochemie; Freie Universitt; Berlin; Germany ; 上海201106; Institut fur Molekularbiologie und Biochemie; Freie Universitt; Berlin; Germany ; 上海201106 ; 上海201106 ; 上海201106; Institut fur Molekularbiologie und Biochemie; Freie Universitt; Berlin; Germany

【中文摘要】 采用體外免疫細(xì)胞培養(yǎng)法,比較了用不同方法制備的灰樹花子實(shí)體和菌絲體多糖的免疫活性。結(jié)果顯示,稀堿提取的灰樹花子實(shí)體多糖(GFAP)能夠顯著提高小鼠淋巴細(xì)胞的轉(zhuǎn)化增殖率,同時(shí)能夠顯著激活小鼠巨噬細(xì)胞,并刺激巨嗜細(xì)胞產(chǎn)生大量一氧化氮(NO)。稀堿提取的灰樹花菌絲體多糖(GMAP)對(duì)小鼠淋巴細(xì)胞與巨嗜細(xì)胞的作用與GFAP相似。熱水提取的灰樹花子實(shí)體多糖(GFP)與菌絲體多糖(GMP)對(duì)小鼠巨噬細(xì)胞有一定的激活作用,并能促使巨嗜細(xì)胞生成NO,但提高小鼠淋巴細(xì)胞轉(zhuǎn)化增殖率的作用均不明顯。

【英文摘要】 The immunocompetence of different kinds of polysaccharide derived from Grifola frondosa was investigated using immunocyte cultivation technique in vitro. The experimental results showed that the proliferation rate of mouse lymphocytes was increased significantly by GFAP (diluted alkali-extracted polysaccharide from the fruitbody of G. frondosa), while the activation of the mouse macrophages was stimulated remarkably, and a great mass of NO was produced by stimulated macrophages; that GMAP (diluted alkali-extracted polysaccharide from the mycelia of G. frondosa) showed the similar effect as that of GFAP on mouse lymphocytes and macrophages; and that the definite activation effect of GFP (hot water-extracted polysaccharide from the fruitbody of G. frondosa) and GMP (hot water-extracted polysaccharide from the mycelia of G. frondosa) on mouse microphages was observed and NO was produced by stimulated macrophages, but the proliferation rate of mouse lymphocytes was not stimulated significa...更多ntly. So it was expected that auxiliary medicine of auti-tumour could be prepared from diluted alkali-extracted G. frondosa polysaccharide through further research  還原

【中文關(guān)鍵詞】 灰樹花多糖; 免疫活性; 淋巴細(xì)胞; 巨噬細(xì)胞; 一氧化氮
【英文關(guān)鍵詞】 Grifola frondosa polysaccharide; Immunocompetence; Lymphocyte; Macrophage; NO
【基金】上海市科委科技攻關(guān)項(xiàng)目"灰樹花的抗癌活性蛋白聚糖生化制品的研制"(編號(hào)013912048)的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-003