邢增濤; 孫芳芳; 劉景圣
農(nóng)業(yè)部食用菌產(chǎn)品質(zhì)量監(jiān)督檢驗(yàn)測試中心(上海) 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開發(fā)實(shí)驗(yàn)室 上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 南京農(nóng)業(yè)大學(xué)資環(huán)學(xué)院; 吉林農(nóng)業(yè)大學(xué)食品科技學(xué)院 上海201106 ; 南京210095 ; 長春130118

【中文摘要】 尖頂羊肚菌液體靜止培養(yǎng)的優(yōu)化培養(yǎng)基為:蔗糖4%,胰蛋白胨0.4%,酵母粉0.15%,天門冬酰胺0.08%,MgSO4·7H2O0.5g/L,KH2PO41.45g/L,Na2HPO4·2H2O0.48g/L,VB125mg/L,CaCl20.013g/L,微量元素溶液1.0mL/L。培養(yǎng)基初始pH7.0,裝量50mL/250mL,接種量2%,培養(yǎng)溫度26℃。培養(yǎng)10d菌絲體生物量可達(dá)12.64g/L。

【英文摘要】 The experimental results showed that the optimized medium for static submerge-cultured (Morchella conica was) composed of sucrose 4%, tryptone 0.4%, yeast extract powder 0.15%, L-asparagine (0.08%, )MgSO_4·7H_2O 0.5g/L,KH_2PO_4 1.45g/L,Na_2HPO_4·2H_2O 0.48g/L,VB_1 25mg/L,CaCl_2 0.013g/L, and element solution 1.0mL/L. And the optimal initial pH, medium capacity, inoculation rate and culture temperature were 7.0, 50mL/250mL, 2% and 26℃ respectively. And the mycelial biomass of Morchellaconica can reach as high as 12.64g/L after inoculation 10 days.

【中文關(guān)鍵詞】 尖頂羊肚菌; 液體靜止培養(yǎng); 生物量; 正交實(shí)驗(yàn)
【英文關(guān)鍵詞】 Morchella conica; Static submerge-culture; Mycelial biomass; Orthogonal test
【基金】上海市農(nóng)業(yè)科學(xué)院發(fā)展基金項目"羊肚菌雙歧桿菌混菌發(fā)酵技術(shù)的研究"的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-008