鄧旺秋; 楊小兵; 李泰輝; 宋斌; 林群英; 周潔瑩; 夏鳳娜
廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室; 廣東省微生物研究所廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室 廣州510070 ; 廣州510070 ; 廣州510070 ; 廣州510070 ; 廣州510070中國(guó)科學(xué)院華南植物研究所廣州510625 ; 廣州510070 ; 廣州510070
【中文摘要】 利用RAPD標(biāo)記對(duì)廣東省8個(gè)草菇主要栽培菌株的親緣關(guān)系和遺傳多樣性進(jìn)行研究,獲得了草菇不同菌株的DNA指紋圖譜。分子聚類結(jié)果表明,8個(gè)供試菌株可分成兩組:菌株V17、V844和粵草01為一組;粵草02、粵草03、粵草04、V5 1和V23為另一組,且同一組內(nèi)的DNA多態(tài)性也有差異。RAPD擴(kuò)增指紋能有效分辨不同菌株的基因型,可為草菇商業(yè)菌株的鑒別提供快速有效的技術(shù)手段,為規(guī)范食用菌菌種生產(chǎn)管理提供科學(xué)依據(jù)。
【英文摘要】 The genetic polymorphic relationship and consanguineous relationship of 8 Volvariella volvacea strains cultivated in Guangdong province of China were studied with the random amplified polymorphic DNA(RAPD) marker in this paper. The DNA fingerprints of different strains showed that there was high genetic diversity among the strains tested. The dendrogram derived from RAPD data by UPGMA suggested that two distinct genetic groups were recognized: one group included V17, V844 and YV01; the other group included YV02, YV03, YV04, V5-1 and V23. And differences of the DNA polymorphism were also found within the same group. While genotypes of different strains could be distinguished by DNA fingerprints produced by RAPD, which could become a fast and effective technique for identifying different strains of Volvariella volvacea and provide the scientific foundation to standardize the production management of edible fungus strains.
【中文關(guān)鍵詞】 草菇; RAPD; 遺傳多態(tài)性; 指紋圖譜
【英文關(guān)鍵詞】 Volvariella volvacea; RAPD; Genetic polymorphism; DNA fingerprint
【基金】廣東省自然科學(xué)基金項(xiàng)目"廣東省安全食用菌產(chǎn)品生產(chǎn)技術(shù)體系的建立與示范"(2002A20507);; "廣東省主產(chǎn)食用菌菌種分子生物學(xué)快速鑒定技術(shù)研究"(2002C20802);; "廣東省毒蘑菇研究"(020469)的部分內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年03期 【DOI】CNKI:SUN:SYJB.0.2004-03-001