何慧; 孫頡; 謝筆鈞
華中農(nóng)業(yè)大學(xué)食品科技學(xué)院; 華中農(nóng)業(yè)大學(xué)食品科技學(xué)院; 華中農(nóng)業(yè)大學(xué)食品科技學(xué)院 武漢430070 ; 武漢430070 ; 武漢430070

【中文摘要】 通過測(cè)定小鼠的肌糖原和肝糖原、采用抗腫瘤藥物體外篩選試驗(yàn)、小鼠腹腔巨噬細(xì)胞吞噬試驗(yàn),分別研究了靈芝水溶性小分子化合物(WSLMFGL,M<10000Da)的抗疲勞、抗腫瘤和免疫調(diào)節(jié)活性。結(jié)果顯示,當(dāng)靈芝水溶性小分子化合物的灌胃劑量超過400mg/kg·d(12d)時(shí),可顯著提高小鼠的肌糖原和肝糖原貯備量,并呈劑量 效應(yīng)關(guān)系;靈芝水溶性小分子化合物對(duì)小鼠Sarco ma 180型腫瘤細(xì)胞的抑制率達(dá)80%;靈芝水溶性小分子化合物的灌胃劑量與小鼠巨噬細(xì)胞吞噬能力的增強(qiáng)及免疫器官的增重存在劑量 效應(yīng)關(guān)系。靈芝水溶性小分子化合物具有抗疲勞、抗腫瘤和免疫調(diào)節(jié)活性。

【英文摘要】 By means of determining the mouse hepatin in muscle and liver, the in-vitro test of anti-tumor medicine and the phagocytosis test of mouse macrophage, the anti-fatigue, anti-tumor and immunomodulating effects of water-soluble lower molecular fraction of Ganoderma lucidum (WSLMFGL) were studied respectively. The experimental results showed that WSLMFGL(Do.≥400mg/kg·d×12d) could significantly increase the reservoir of mouse hepatin in muscle and liver in a dose-dependant manner, that WSLMFGL's inhibition ratio to mouse Sarcoma-180 tumor cells was as high as 80%, and that WSLMFGL could increase the phagocytosis ability of the mice and the weight of mouse immune organs in a dose-dependant manner. So WSLMFGL was considered to have the bioactivities of anti-fatigue, anti-tumor and immunomodulation.

【中文關(guān)鍵詞】 靈芝水溶性小分子化合物; 生物活性; 抗疲勞; 抗腫瘤; 免疫調(diào)節(jié)
【英文關(guān)鍵詞】 Water-soluble lower molecular fraction of Ganoderma lucidum (WSLMFGL); Bioactivity; Anti-fatigue; Anti-tumor; Immunomodulation
【基金】華中農(nóng)業(yè)大學(xué)科技創(chuàng)新基金資助項(xiàng)目"靈芝肽生物活性及其構(gòu)效關(guān)系研究"(項(xiàng)目編號(hào):52204-02038)的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年03期 【DOI】CNKI:SUN:SYJB.0.2004-03-003