劉傳富; 皇傳華; 黃春燕; 單洪濤; 趙傳麟
濟南市農(nóng)業(yè)科學研究所; 濟南市農(nóng)業(yè)科學研究所; 山東省農(nóng)科院植保所; 山東省農(nóng)科院植保所; 濟南市農(nóng)業(yè)科學研究所 濟南250023 ; 濟南250023 ; 濟南250100 ; 濟南250100 ; 濟南250023
【中文摘要】 研究結(jié)果表明,培養(yǎng)雞腿蘑液體菌種的適宜培養(yǎng)基配方為蔗糖2%,玉米粉3%,麥麩4%,酵母粉0.5%,KH2PO40.25%,MgSO4·7H2O0.1%,pH6.2;最適培養(yǎng)條件是250mL三角瓶裝液量60mL,接種量5%,搖床轉(zhuǎn)速120r min,培養(yǎng)6d。利用液體菌種擴繁固體生產(chǎn)種,可以明顯縮短菌種生產(chǎn)周期。
【英文摘要】 The experimental results showed that the optimum medium of Coprinus comatus liquid spawn was sucrose 2%,corn powder 3%,wheat bran 4%,yeast powder 0.5%,KH_2PO_4 0.25%,MgSO_4·7H_2O 0.1%,and that the optimum culture conditions were as follows: initial pH6.2,medium capacity 60mL/250mL flask, rocking bed rotation rate 120r/min, inoculation rate 5% and the culture period of about 6 days. And the culture period will be shortened significantly if liquid spawn was used to breed the solid culture spawn.
【中文關(guān)鍵詞】 雞腿蘑; 液體菌種; 培養(yǎng)條件; 正交試驗
【英文關(guān)鍵詞】 Coprinus comatus; Liquid spawn; Culture condition; Orthogonal test
【基金】山東省濟南市科技局"食用菌菌種引進選育及研究"[濟科合字(2001)第70-1號]的部分內(nèi)容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2004年02期 【DOI】CNKI:SUN:SYJB.0.2004-02-005