范雷法; 潘慧娟; Soccol CR; Pamdey A
浙江省農(nóng)業(yè)科學(xué)院園藝所; 浙江省農(nóng)業(yè)科學(xué)院園藝所; Laboratory of Biotechnological Process; Federal University of Parana; Biotechnology Division; Regional Research Laboratory 杭州310021 ; 杭州310021 ; Curitiba-PR; 81531-970 Brazil ; Trivandrum-695 019; India

【中文摘要】 通過(guò)深層發(fā)酵培養(yǎng)姬松茸菌絲體,濾取菌絲體后獲得菌絲體培養(yǎng)液,經(jīng)沉淀離心收集其胞外粗多糖,再用蒸餾水溶解,取可溶性部分。對(duì)接種S 180的小白鼠24h后腹腔注射給藥,連續(xù)10d,劑量為10mg kg·d。5周后剝?nèi)×鰤K,與對(duì)照組比較瘤重。結(jié)果發(fā)現(xiàn),姬松茸深層發(fā)酵生產(chǎn)的胞外多糖對(duì)小鼠接種的腹水瘤S 180有較強(qiáng)的抗性,腫瘤抑制率達(dá)到44.53%,完全抑制率達(dá)到50%。

【英文摘要】 Ab-EPS was produced by submerged fermentation and extracted from the liquid media by ethanol precipitation and centrifugation. After removing the insoluble part in water, the Ab-EPS was applied to mice inoculated with tumor cell of S-180 24h later by intraperitoneal injection for 10 consecutive days at the dose of 10 mg/kg·d. Five weeks later, the tumor weight was evaluated and compared with that of the control group. Ab-EPS showed a higher antitumor activity against S-180 grafted in mice with the tumor inhibition rate being 44.53% and the complete inhibition rate being 50%.

【中文關(guān)鍵詞】 姬松茸; 胞外多糖; 抗腫瘤活性; S180
【英文關(guān)鍵詞】 Agaricus blazei; Exopolysaccharide; Antitumor activity; S-180
【基金】巴西科技部資助項(xiàng)目"姬松茸胞外多糖的優(yōu)化生產(chǎn)和抗腫瘤研究"(CNPq:140165 2000-2)的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年02期 【DOI】CNKI:SUN:SYJB.0.2004-02-010