李三暑; 林新堅(jiān); 陳惠成; 雷錦桂; 林勇; 江枝和
福建省農(nóng)科院土壤肥料研究所; 福建省農(nóng)科院土壤肥料研究所; 福建省農(nóng)科院土壤肥料研究所; 福建省農(nóng)科院土壤肥料研究所; 福建省農(nóng)科院土壤肥料研究所; 福建省農(nóng)科院土壤肥料研究所 福州350013 ; 福州350013 ; 福州350013 ; 福州350013 ; 福州350013 ; 福州350013

【中文摘要】 以 4個(gè)姬松茸菌株和 2個(gè)雙孢蘑菇菌株為材料 ,用 2 0個(gè)隨機(jī)引物對(duì)它們進(jìn)行RAPD擴(kuò)增。通過(guò)對(duì)供試菌株遺傳相似系數(shù)的估算和系統(tǒng)聚類分析 ,構(gòu)建姬松茸和雙孢蘑菇遺傳相關(guān)樹(shù)狀聚類圖譜。結(jié)果表明 ,進(jìn)行RAPD擴(kuò)增的 2 0個(gè)隨機(jī)引物中 ,有 12個(gè)能產(chǎn)生三種帶型。當(dāng)遺傳相似系數(shù)升至 0 .8713時(shí) ,這 6個(gè)菌株被分為 3類 ,第一類為菌株 18和 2 6 ,第二類為菌株 13和 12 ,第三類為菌株 33和 4 5。

【英文摘要】 Four strains of Agaricus blazei and two strains of Agaricus bisporus were amplified by RAPD (Random Amplified Polymorphic DNA) technique with 20 random primers. A dendrogram was generated through the assessment of the similarity coefficient of these six strains tested and systematic clustering analysis. The result showed that among 20 primers tested, 12 primers could produce three band types of DNA fragments. When the similarity coefficient raised to 0.8713, six strains tested were divided into three classes. The first class included strains 18 and 26, the second class included strains 12 and 13 and the third class included strains 33 and 45.

【中文關(guān)鍵詞】 姬松茸; 雙孢蘑菇; RAPD
【英文關(guān)鍵詞】 Agaricus blazei; Agaricus bisporus; RAPD(Random Amplified Polymorphic DNA)

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2002年03期 【DOI】CNKI:SUN:SYJB.0.2002-03-000