蘭瑞芳; 林少琴; 林玉滿; 馮珊
福建師范大學(xué)實(shí)驗(yàn)中心; 福建師范大學(xué)實(shí)驗(yàn)中心; 福建師范大學(xué)實(shí)驗(yàn)中心; 福建省衛(wèi)生學(xué)校 福州350007 ; 福州350007 ; 福州350007 ; 福州350001

【中文摘要】 從杏鮑菇、雙孢蘑菇、美味側(cè)耳、茶薪菇、鳳尾菇和香菇子實(shí)體中提取漆酶 ,用分光光度法測(cè)定酶活 ,發(fā)現(xiàn)杏鮑菇漆酶酶活最高。實(shí)驗(yàn)結(jié)果表明 ,杏鮑菇漆酶是一種較穩(wěn)定的高溫酶 ,其最適 pH為 3.0 ,最適溫度 6 0℃ ;Pb2 + 、K+ 對(duì)酶活有較大的激活作用 ,而Fe3 + 、Fe2 + 則對(duì)酶活有較強(qiáng)的抑制作用

【英文摘要】 The laccase was extracted from fresh fruitbodies of Pleurotus eryngii, Agaricus bisporus, Pleurotus sapidus, Agrocybe cylindracea, Pleurotus pulmonarius and Lentinula edodes. It was proved in our experiments that the laccase activity ofPleurotus eryngii determined with spectrophotometry was the highest. The experiment results also indicated that the laccase was stable under high temperature, its optimal temperature and pH were 60℃ and 3.0 respectively,and that its activity was stimulated significantly by the metal ions of Pb 2+ and K +, but inhibited by the metal ions of Fe 3+ and Fe 2+ .

【中文關(guān)鍵詞】 漆酶; 杏鮑菇; 生物學(xué)特性
【英文關(guān)鍵詞】 Laccase; Pleurotus eryngii; Biological characteristic
【基金】福建省教委WX-189項(xiàng)目資助

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2002年02期 【DOI】CNKI:SUN:SYJB.0.2002-02-003