宋思揚(yáng); 曾偉; 陳融; 王澤生; 蘇文金
廈門大學(xué)腫瘤細(xì)胞工程國家專業(yè)實(shí)驗(yàn)室!廈門361005; 廈門大學(xué)腫瘤細(xì)胞工程國家專業(yè)實(shí)驗(yàn)室!廈門361005福建省輕工業(yè)研究所; 福州350005; 廈門大學(xué)腫瘤細(xì)胞工程國家專業(yè)實(shí)驗(yàn)室!廈門361005; 福建省輕工業(yè)研究所!福州350005; 廈門大學(xué)腫瘤細(xì)胞工程國家專業(yè)實(shí)驗(yàn)室!廈門361005

【中文摘要】 利用 2 0個十堿基隨機(jī)引物 ,在基因組水平上對雙孢蘑菇 (Agaricusbisporus)的優(yōu)質(zhì)低產(chǎn)型 (G型 )及低質(zhì)高產(chǎn)型 (H型 )菌株進(jìn)行了RAPD差異顯示 ,獲得一個與雙孢蘑菇子實(shí)體品質(zhì)相關(guān)的大小約為 2 0 0 0bp的差異DNA片段 ,將其克隆 ,并應(yīng)用點(diǎn)雜交及RFLP技術(shù)對其區(qū)分兩類菌株的有效性進(jìn)行了檢驗(yàn)。這為進(jìn)一步建立特異性探針或PCR引物 ,從分子水平上提高雙孢蘑菇菌種性狀早期預(yù)測的準(zhǔn)確性奠定了基礎(chǔ)

【英文摘要】 With 20 ten-mers random primers, the genomic differences between two types of Agaricus bisporus strain-good quality with low yield (G type) vs. poor quality with high yield (H type), were displayed by Random Amplified Polymorphic DNA (RAPD) technique. A 2000bp specific DNA fragment correlated to the quality of Agaricus bisporus fruitbody was obtained and cloned. Then dot hybridization and RFLP technique were used to determine the effectiveness of distinguishing two types of A. bisporus strains. And it was considered that our study had laid a foundation for further establishing a specific probe or PCR primer and for improving the accuracy of early predicting the characteristics of A. bisporus strains at molecular level.

【中文關(guān)鍵詞】 雙孢蘑菇; RAPD; 子實(shí)體品質(zhì); DNA克隆; RFLP
【英文關(guān)鍵詞】 Agaricus bisporus; RAPD; Fruitbody; Cloning; RFLP
【基金】福建省自然科學(xué)基金資助項目!(C982 0 0 0 5 )

【文獻(xiàn)出處】 食用菌學(xué)報,ACTA EDULIS FUNGI,編輯部郵箱,2000年03期 【DOI】CNKI:SUN:SYJB.0.2000-03-002