陳明杰; 余智晟; 凌霞芬; 王南; 譚琦; 呂作舟; 潘迎捷
農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室!上海市農(nóng)業(yè)遺傳育種重點開放實驗室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海201106; 華中農(nóng)業(yè)大學(xué)微生物系應(yīng)用真菌室!武漢430070; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室!上海市農(nóng)業(yè)遺傳育種重點開放實驗室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 上海2011

【中文摘要】 利用現(xiàn)代分子生物學(xué)技術(shù)擴(kuò)增獲得草菇組織分離物與草菇出發(fā)菌株的RAPD和ITS的DNA指紋圖譜 ,以此估算兩者的DNA相似系數(shù) ,并構(gòu)建了遺傳相關(guān)聚類圖。結(jié)果表明 ,兩者存在著一定的遺傳差異。

【英文摘要】 The amplified DNA fingerprints from RAPD and ITS of Volvariella volvacea tissue isolates and parental strains were obtained by modern molecular biological techniques. The DNA similarity coefficients of V. volvacea strain and tissue isolate were estimated and cluster dendrogram was constructed based on the DNA fingerprints. The results showed that genetic variation existed between these two strains.

【中文關(guān)鍵詞】 草菇; 組織分離; 遺傳變異
【英文關(guān)鍵詞】 Volvariella volvacea; Tissue isolate; Genetic variatio(
【基金】上海市農(nóng)委科技興農(nóng)重點攻關(guān)項目

【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2000年02期 【DOI】CNKI:SUN:SYJB.0.2000-02-000