王南; 鮑大鵬; 黃禮濱; 譚琦; 潘迎捷
農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室; 農(nóng)業(yè)部食用菌遺傳育種重點開放實驗室 上海市農(nóng)業(yè)遺傳育種重點開放實驗室 上海市農(nóng)業(yè)科學院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點開放實驗室 上海市農(nóng)業(yè)科學院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點開放實驗室 上海市農(nóng)業(yè)科學院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點開放實驗室 上海市農(nóng)業(yè)科學院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點開放實驗室 上海市農(nóng)業(yè)科學院食用菌研究所 上海 201106
【中文摘要】 以不同來源的柱狀田頭菇(Agrocybe aegerita)為研究對象,就不同的碳氮比、pH值以及培養(yǎng)溫度對菌絲生長速度和結(jié)實性的影響進行了比較研究,并從同工酶角度分析比較了各菌株的親緣關(guān)系。研究結(jié)果表明,不同來源的柱狀田頭菇所要求的最佳培養(yǎng)條件不盡相同。一般情況下,菌絲在碳氮比為10:1～80:1時均可生長,碳氮比為10:1時,菌絲生長最快,且易結(jié)實;柱狀田頭菇可生長并結(jié)實的pH值為4～12,最適宜結(jié)實的pH值為6;25℃時柱狀田頭菇菌絲生長速度最快,而20～25℃比較適合其結(jié)實。同工酶分析表明,柱狀田頭菇菌絲中過氧化物同工酶活性不高,酶帶單調(diào),不適宜用做檢測遺傳多樣性的生化標記;酯酶同工酶有一定的活性,可以從相似系數(shù)上分析不同菌株的親緣關(guān)系。
【英文摘要】 Agrocybe aegerita strains from different countries and areas were collected. The effect of carbon nitrogen ratio, pH and temperature on mycelial growth and fecundity of A. aegerita. strains was compared. Meanwhile the isozyme tests were applied to analyze the relationship of various strains. The results indicated that the optimal culture conditions were not all the same for A. aegerita strains selected from different sources. Normally, the C/N and pH range suitable for mycelial growth and fruitbody formation were 10:1 - 80:1 and pH4 - 12 resp. , while the optimum C/N and temperature for mycelial growth were 10:1 and 25℃ resp. and optimum pH and temperature for fruitbody formation were pH 6 and 20-25℃ resp. . According to the results of isozyme tests, the per-oxidase isozyme activity of the mycelia was low and the isozyme bands were dull. So, peroxidase isozyme could not be used as biochemical mark for examining genetic diversity. Neverthless, the esterase isozyme activity of mycelia wa...更多s higher and the isozyme bands were more abundant, so it could be applied to analyze the relationship of different A. aegerita strains. 還原
【中文關(guān)鍵詞】 柱狀田頭菇; 培養(yǎng)條件; 同工酶
【英文關(guān)鍵詞】 Agrocybe aegerita; Culture condition; Isozyme
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1999年03期 【DOI】CNKI:SUN:SYJB.0.1999-03-000