曾大興; 謝和
貴州大學(xué)南區(qū)園藝系; 貴州大學(xué)南區(qū)園藝系 貴陽 550025 ; 貴陽 550025

【中文摘要】 在適宜培養(yǎng)條件下,振蕩培養(yǎng)4～5d的雞縱菌(Termitomyces albuminosus)和粗柄雞■菌(T.robustus)幼嫩菌絲,經(jīng)過濾洗滌收集,以0.6mol/L KCl為滲透壓穩(wěn)定劑,用1％纖維素酶加0.5％蝸牛酶的混合酶液處理,于28℃酶解3～4h,可獲大量原生質(zhì)體,產(chǎn)量達2.82×106～3.24×106/mL。但所形成的原生質(zhì)體再生能力較差,再生率僅0.6％～1.2％。本文還詳細研究了菌齡、酶系統(tǒng)、酶解溫度和時間等多種因素對這兩種雞縱菌原生質(zhì)體形成和再生的影響。

【英文摘要】 The mycelia shaking- cultured 4-5 days were gathered after being filtered and rinsed. When the mycelia were treated with a combined enzyme system composed of 1 % cellulase and 0.5% glusulase with 0. 6mol/L KC1 being used as osmotic stabilizer at 28℃ for 3-4h, high yield of protoplast (2. 82 - 3. 24×106/mL) was obtained from Termitomyces albuminosus and T. robustus. The protoplast regeneration rate of T. albuminusus and T. robustus was 0.6% - 1. 2% . While the effects of hyphal age, lytic enzyme system, osmotic stabilizer, enzy-molysis temperature and time on the preparation and regeneration of protoplast were also discussed in this paper.

【中文關(guān)鍵詞】 雞■菌; 粗柄雞■菌; 原生質(zhì)體形成和再生
【英文關(guān)鍵詞】 Termitamyces albuminosus; T. robustus; Preparation and regeneration of protoplast
【基金】貴州省科學(xué)基金.項目編號:95096B—138

【文獻出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1999年02期 【DOI】CNKI:SUN:SYJB.0.1999-02-001