姜文俠; 孫武岳; 馬琳; 呂呈剛; 易雙全
天津市工業(yè)微生物研究所; 天津市工業(yè)微生物研究所; 天津市工業(yè)微生物研究所; 天津市工業(yè)微生物研究所; 天津市工業(yè)微生物研究所 天津 300221 ; 天津 300221 ; 天津 300221 ; 天津 300221 天津輕工業(yè)學(xué)院畢業(yè)實(shí)習(xí)生 ; 天津 300221 天津輕工業(yè)學(xué)院畢業(yè)實(shí)習(xí)生
【中文摘要】 試驗(yàn)研究了冬蟲夏草(Cordyceps sinensis)蝙蝠蛾擬青霉(Paecilomyces hepiali Chen et Dai)菌絲體的發(fā)酵條件,其適宜接種量為2.5%~5.0%(v/v),最佳菌齡為3d,適宜的初始pH為5.0~7.5,發(fā)酵周期5d,發(fā)酵過(guò)程中對(duì)通氣量的要求較高。以20m3發(fā)酵罐培養(yǎng),菌絲體收率為2.5%,其D-甘露醇含量8.3%,腺苷含量0.24%。
【英文摘要】 The mycelial fermentation conditioas of Paecilomyces hepiali Chen et Dai of Cordyceps sinensis were studied in our last. The mycelia were cultured at 24℃ on a rotary shaker at 120 r/min for 5 days. It was proved that the suitable initial pH of the medium was 5.0 - 7.5, the inoculation ratio was 2.5% -5.0%(v/v) and the optimal spawn age was 3 days, and that more attention should be paid to the aeration during the fermentation process. Fermented in 20m3 fermentor, the yield of mycelia was 2. 5% .among which the content of D- mannitol in the dried mycelium was 8.3% and the content of adenosine was 0. 24% .
【中文關(guān)鍵詞】 蝙蝠蛾擬青霉; 菌絲體發(fā)酵; 冬蟲夏草
【英文關(guān)鍵詞】 Paecilomyces hepiali ; Mycelial fermentation; Cardyceps sinensis
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1999年02期 【DOI】CNKI:SUN:SYJB.0.1999-02-008