賈建航; 劉振岳; 泰麗芳; 劉國(guó)振; 張偉; 李莉云; 李小兵
河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 河北農(nóng)業(yè)大學(xué)細(xì)胞工程育種課題組; 中國(guó)科學(xué)院遺傳研究所 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 保定071001 ; 北京100101

【中文摘要】 采用氯化芐方法提取香菇總DNA,用Gene Pulser Ⅱ電擊系統(tǒng)將香菇DNA導(dǎo)入平菇單核原生質(zhì)體.通過(guò)香菇DNA的導(dǎo)入,利用基因互補(bǔ)原理獲得了能出菇的轉(zhuǎn)化子1-1和13-48,并對(duì)轉(zhuǎn)化子進(jìn)行了拮抗試驗(yàn)、菌絲生長(zhǎng)速度分析、酯酶同工酶分析和RAPD分析.

【英文摘要】 Abstract The total DNA of Lentinula edodea isolated by the method of benzyl chloride was introduced into protoplasts of monokaryotic mycelia of P. oatreatua with Bio-Rad Gene Pulser n electroporation system. Optimal electroporation condition was selected in this study. Transfor-mants 1-1 and 13-48 with clamp connection were obtained and formed fruitbodies through principle of genie complementation after introducing L. edodea DNA. While the antagonism reaction test and the mycelium growth rate, esterase isozyme and RAPD analysis of the transformants were also carried out in this study.

【中文關(guān)鍵詞】 香菇; 平菇; 電擊; 轉(zhuǎn)化子
【英文關(guān)鍵詞】 Lentinula edodes; Pleurotus ostreatus; Electroporation; Transformant
【基金】河北省自然科學(xué)基金資助

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-001