姜性堅(jiān); 王利群; 熊輝; 張曉元; 石正軍
湖南省食用菌研究所; 湖南省食用菌研究所; 湖南省食用菌研究所; 湖南農(nóng)業(yè)大學(xué)園藝系; 湖南農(nóng)業(yè)大學(xué)園藝系 長(zhǎng)沙410006 ; 長(zhǎng)沙410006 ; 長(zhǎng)沙410006 ; 長(zhǎng)沙410128 ; 長(zhǎng)沙410128

【中文摘要】 本文應(yīng)用垂直平板聚丙烯酰胺不連續(xù)電泳,對(duì)金針菇(Flammulina velutipes)12個(gè)栽培菌株的菌絲體和其中9個(gè)菌株的子實(shí)體進(jìn)行酯酶同工酶比較;并對(duì)金針菇子實(shí)體發(fā)育不同時(shí)期和不同部位酯酶同工酶的變化進(jìn)行分析.結(jié)果表明,金針菇菌株間酯酶同工酶譜變化較大,且在子實(shí)體階段比菌絲體階段表現(xiàn)更為明顯;同一菌株在于實(shí)體不同發(fā)育時(shí)期和不同部位酶帶數(shù)量穩(wěn)定,但顯色強(qiáng)弱有明顯的變化.子實(shí)體伸長(zhǎng)期和成熟階段的菌蓋部位酶帶顯色最深.

【英文摘要】 The comparison of esterase isozyme in mycelium of 12 Flammulina velutipes strains and fruitbody of 9 of the 12 F.velutipes strains and the analysis of changes in esterase isozyme at different part of fruitbody during different development periods were carried out in this study with orthogonal slab polyacrylamide gel electrophoresis (OSPAGE). The results showed that there were great differences of esterase isozyme zymogram among strains, and that the difference was more significant at fruitbody phase, while the no. of esterase isozyme band was stable at different part of fruitbody during various development periods for the same strain. But the color of the band changed significantly and the most dark-coloured band appeared at elongation period of the fruitbody and maturity period of the pileus.

【中文關(guān)鍵詞】 金針菇; 菌株; 酯酶同工酶
【英文關(guān)鍵詞】 Flammulina velutipes; Strain; Esterase isozyme

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-007