林樹錢; 沈國華; 程立佳; 游蘭英
福建省三明市真菌研究所; 福建省三明市真菌研究所; 福建省三明市真菌研究所; 福建省三明市真菌研究所 三明365000 福建省醫(yī)學(xué)科學(xué)研究所 ; 三明365000 漳州市科委 ; 三明365000 ; 三明365000 廈門市醫(yī)藥研究所
【中文摘要】 本文研究了層臥孔菌[Fomitiporia punctata(Fr.)Murr.]的菌種分離、培養(yǎng)基配方.探索了培養(yǎng)條件,并投入中試規(guī)模的批量生產(chǎn).層臥孔菌最適培養(yǎng)基為麥麩-蔗糖-酵母粉.中試生產(chǎn)溫度控制在28±2℃,最適pH為5.5~6.0,最佳接種量為15%~20%.通氣量為1:0.5(v/■v/min).培養(yǎng)80h左右.即可得到層臥孔菌菌絲體培養(yǎng)物.
【英文摘要】 Fomitiporia punctata (Fr. ) Murr. is one of the medicinal fungi used to treat coronary heart disease in Fujian Province. F. punctata strain was isolated, its medium composition and cultivation conditions in submerged culture were studied in order to produce the mycelia at pilot-production-scale. It was demonstrated that the optimum temperature, pH, inoculation rate and medium content per bottle were 28 + 2C, 5.56.0, 15%20% and 1 : 0.5 (v/v/min) resp. in pilot production, and that mycelium culture could be got after 80h cultivation.
【中文關(guān)鍵詞】 層臥孔菌; 深層培養(yǎng); 菌絲體培養(yǎng)物
【英文關(guān)鍵詞】 Fomitiporia punctata (Fr. ) Murr; Submerged culture; Mycelium culture
【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,1997年04期 【DOI】CNKI:SUN:SYJB.0.1997-04-008