楊岱筠; 張丕奇; 鄭美媛; 方自若
黑龍江省科學(xué)院應(yīng)用微生物研究所; 黑龍江省科學(xué)院應(yīng)用微生物研究所; 黑龍江省科學(xué)院應(yīng)用微生物研究所; 黑龍江省科學(xué)院應(yīng)用微生物研究所 哈爾濱 150010 ; 哈爾濱 150010 ; 哈爾濱 150010 ; 哈爾濱 150010

【中文摘要】 利用原生質(zhì)體技術(shù)獲得的白色金針菇原生質(zhì)體再生菌株,根據(jù)細(xì)胞核的分布與數(shù)目以及鎖狀聯(lián)合的有無(wú),選出25株單核菌株,對(duì)峙試驗(yàn)表明,這些單核株分為核基因型不同的兩種類型。兩種類型的單核株和始發(fā)雙核菌株的菌落形態(tài)、生長(zhǎng)速度、胞外漆酶活性和藥物抗性等性狀指標(biāo)均有明顯差異,表明生成原生質(zhì)體的過(guò)程中,所再生的單核株發(fā)生了變化。

【英文摘要】 The regenerated strain of white Flammulina velutipes protoplast can be obtained through protoplast technique. According'to the distribution, the number of nuclei and the presence of clamp connection in cells, 25 strains have been distinguished as single-nucleus strains. The confrontation test indicates that these single-nucleus strains which can be classified as two nucleus-genotypes, are obviously different from the original two-nucleus. strains in morphology, growth rate, ELA and resistance to drugs. The regenerated single-nucleus strains are showed to have altered in the process of protoplastization.

【中文關(guān)鍵詞】 白色金針菇; 單核菌株; 基因型; 放線菌酮; 吖啶黃; 漆酶
【英文關(guān)鍵詞】 White Flammulina velutipes; Single nucleus strin Genotype; Actidione Fluka (ACT); Acriflavinc (ACR); Laccase

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1995年04期 【DOI】CNKI:SUN:SYJB.0.1995-04-002