肖光輝; 吳德喜; 李青
湖南省園藝研究所!長(zhǎng)沙410125; 湖南省園藝研究所!長(zhǎng)沙410125; 湖南省園藝研究所!長(zhǎng)沙410125
【中文摘要】 在碳素含量為2%、氮素含量為0.06%的條件下,采用液體靜止培養(yǎng)方法,研究了4個(gè)猴頭菌菌株對(duì)碳、氮營(yíng)養(yǎng)的利用。結(jié)果表明,菌絲營(yíng)養(yǎng)生長(zhǎng)對(duì)有機(jī)氮的利用比對(duì)無(wú)機(jī)氮強(qiáng),有機(jī)氮中,其對(duì)復(fù)合氮素如蛋白胨、干酪素、酵母膏的利用比對(duì)單一氮素如尿素、谷氨酰胺強(qiáng)。無(wú)機(jī)氮中,其對(duì)銨態(tài)氮利用較強(qiáng),硝態(tài)氮基本上不能被利用。猴頭菌對(duì)碳素營(yíng)養(yǎng)的利用廣泛,在多糖中對(duì)糊精的利用最好,其次是可溶性淀粉,對(duì)纖維素的利用較差。單糖中的甘露糖、葡萄糖,雙糖中的麥芽糖、蔗糖為較理想的碳源。以葡萄糖為碳源,硫酸銨為氮源時(shí),猴頭菌菌絲生長(zhǎng)的適宜C/N比為6~21∶1,最適C/N比為15∶1。當(dāng)C/N比為15∶1、培養(yǎng)基含氮量為0.18%~0.22%時(shí),菌絲生長(zhǎng)旺盛,對(duì)營(yíng)養(yǎng)的利用比較合理。
【英文摘要】 C- and N-nutrition utilization of 4 strains of Hericium erinaceus was studied in quiet liquid culture with 2% C-nutrition and 0.06 % N-nutrition. The results showed that organic nitrogens were better utilized than inorganic nitrogens by Hericium erinaceus mycelia. Among organic nitrogens, complex nitrogens, such as peptone, dry casein and yeast extract were better utilized than simple nitrogens, such as urea and glutamine by the mycelia. Among inorganic nitrogens, ammonium nitrogen was well utilized, but nitrate nitrogen was hardly utilized. Hericium erinaceus mycelia utilized carbon nutrition widely. Dextrin was the best utilized polysaccharide, secondly was soluble starch and the last was cellulose. Mannose and glucose were well utilized monosaccharides, while maltose and sucrose were well utilized disaccharides. The optimum C/N-ratio for Hericium erinaceus mycelia growth in liquid culture was 6-21:1 when glucose and (NH4)2SO4 were used as C- and N-nutrition resp.. At the C/N-ratio o...更多f 15:1 and N-content of 0.18%-0.22%, the mycelia of Hericium erinaceus grew very well and the nutrition of the substrate was better used. 還原
【中文關(guān)鍵詞】 猴頭菌; 碳素營(yíng)養(yǎng); 氮素營(yíng)養(yǎng); C/N比
【英文關(guān)鍵詞】 Hericium erinaceus; C-nutrition; N-nutrition; C/N-ratio
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1995年02期 【DOI】CNKI:SUN:SYJB.0.1995-02-005