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    混菌發(fā)酵油菜稈氮源對(duì)靈芝木質(zhì)素酶系的影響


    【發(fā)布日期】:2010-05-14

    張丁倩; 柯麗霞; 趙文科; 吳義祥; 周科
    安徽師范大學(xué)生命科學(xué)學(xué)院安徽省高校生物環(huán)境與生態(tài)安全省級(jí)重點(diǎn)實(shí)驗(yàn)室

    【中文摘要】 研究了豆渣和酒石酸銨(濃度分別為1%、2%、3%、4%和5%),對(duì)靈芝和產(chǎn)朊假絲酵母混菌固態(tài)發(fā)酵油菜秸稈產(chǎn)木質(zhì)素降解酶系的影響。結(jié)果表明,與對(duì)照相比,1%、2%、3%和5%的豆渣組中的漆酶、錳過氧化物酶和木質(zhì)素過氧化物酶酶活性均未受顯著性影響,而在4%豆渣組中,這3種酶的活性顯著提高,分別為440.33 U·L-1、473.33 U·L-1和98.49 U·L-1,分別是對(duì)照的2.03倍,2.16倍和2.86倍。與對(duì)照相比,1%、2%、3%、4%和5%的酒石酸銨組中,3種木質(zhì)素降解酶的活性均無(wú)顯著性影響,因此有機(jī)氮豆渣好于酒石酸銨,其最佳濃度為4%。

    【英文摘要】 Effects of the organic bean dregs powder and ammonium tartrate dibasic at the concentration of 1%,2%,3%,4% and 5%on the production of ligninolytic enzyme system under solid-state co-fermentation of Ganoderma lucidum and Candida utilis in cole stalk medium were studied.The results showed that compared with the control team,it did not affect the activities of laccase,manganese peroxidase and lignin peroxidase significantly,when nitrogen sources was bean dregs powder of 1%,2%, 3%and 5%.However the activities of laccase,manganese peroxidase and lignin peroxidase were increased significantly when nitrogen sources was 4%bean dregs powder,and the activities of three enzymies were 440.33 U·L-1,473.33 U·L-1 and 98.48 U·L-1 respectively which were 2.03,2.16 and 2.86 times compared to the control team.Compared with the control team,it did not affect the activities of laccase,manganese peroxidase and lignin peroxidase significantly,when nitrogen sources was 1%, 2%,3%,4%and 5%ammonium tart...更多rate dibasic.Organic bean dregs powder was better than the ammonium tartrate dibasic as nitrogen sources,whose best concentration was 4%.  還原

    【中文關(guān)鍵詞】 混菌固態(tài)發(fā)酵; 油菜秸稈; 氮源; 木質(zhì)素降解酶系
    【英文關(guān)鍵詞】 Solid-state co-fermentation; Cole stalk; Nitrogen sources; Ligninolytic enzyme system
    【基金】安徽省教育廳自然基金重點(diǎn)項(xiàng)目(j2008A135);; 安徽師范大學(xué)博士科研啟動(dòng)基金

    【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年01期 【DOI】CNKI:SUN:ZSYJ.0.2010-01-016

     
     
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