黃龍花; 楊小兵; 張智; 胡惠萍; 張一帆; 吳清平
廣東粵微食用菌技術(shù)有限公司; 廣東省微生物研究所; 廣東省菌種保藏與應(yīng)用重點(diǎn)實(shí)驗(yàn)室

【中文摘要】 針對(duì)11個(gè)本所保藏靈芝屬菌株及9個(gè)靈芝屬野生菌株的ITS序列,應(yīng)用特異引物進(jìn)行PCR擴(kuò)增,對(duì)其產(chǎn)物進(jìn)行測(cè)序,并從GenBank上下載2條赤芝(Ganoderma luncidum)、1條黑紫靈芝(Ganoderma japonicum)和1條無(wú)柄紫靈芝(Ganoderma mastoporum)的ITS序列,以猴頭菇(Hericium erinaceum)為外類群,用NJ(Neighbor-Joining)法構(gòu)建系統(tǒng)發(fā)育樹,結(jié)果表明,20個(gè)靈芝屬菌株聚為4個(gè)簇,其中赤芝組和樹舌亞屬的菌株各自聚為1組,其組內(nèi)同源性均達(dá)99.7%以上,紫靈芝組的菌株分成2組:無(wú)柄紫靈芝和黑紫靈芝。系統(tǒng)發(fā)育分析也表明僅根據(jù)形態(tài)學(xué)特征難以將靈芝屬菌株進(jìn)行有效的分類,利用分子生物學(xué)的技術(shù)手段對(duì)靈芝菌株進(jìn)行分類是一種更有效的方法,ITS序列分析在近緣種的分類鑒定上很有價(jià)值。

【英文摘要】 ITS sequence analysis of 11 preserved strains and 9 wild strains of Ganoderma spp.were investigated in this study. ITS sequences were produced from 20 strains of Ganoderma spp.by cloning sequencing of PCR products.Data were combined with ITS sequences from 6 related strains,including Ganoderma luncidum,Ganodermajaponicum,Ganoderma mastoporum and Hericium erinaceum,obtained from GenBank to construct a phylogenetic tree using the Neighbor-joining method with Hericium erinaceum as the outgroups.Four distinct groups were identified including the sect.Ganoderma,the sect.subgenus Elfvingia, the sect.Phaeonema.Similarity value among sects Ganoderma and subgenus Elfvingia strains were>99.7%.Analysis of molecular data is a more effective and useful approach for studying the taxonomy of Ganoderma,and for establishing phylogenefic relationships within the genus,compared to methods based on morphology of fruiting body.

【中文關(guān)鍵詞】 靈芝屬; ITS序列; 系統(tǒng)發(fā)育樹; 鑒定
【英文關(guān)鍵詞】 Ganoderma; ITS sequences; Phylogenetic tree; Identification
【基金】廣州市科技支撐計(jì)劃項(xiàng)目(2008Z1-E011);; 國(guó)際科技合作項(xiàng)目(2009DFA30830)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年01期 【DOI】CNKI:SUN:ZSYJ.0.2010-01-021