黃達(dá)明; 劉芹; 張志才; 張玲; 仲媛; 趙繼友
江蘇大學(xué)食品與生物工程學(xué)院

【中文摘要】 探索樹舌發(fā)酵產(chǎn)生高活性的碳酸酐酶抑制劑的營(yíng)養(yǎng)需求。試驗(yàn)以抑制碳酸酐酶活性為指標(biāo),通過單因素試驗(yàn)篩選出適宜碳源、氮源及復(fù)合營(yíng)養(yǎng)因子,通過正交試驗(yàn)進(jìn)一步觀察了各組分之間的相互作用。試驗(yàn)結(jié)果表明,樹舌發(fā)酵合成碳酸酐酶抑制劑的較為適合的培養(yǎng)基為:果糖20 g.L-1、NaNO3 2 g.L-1、馬鈴薯173 g.L-1、(NH4)2SO4 1.3 g.L-1。使用該培養(yǎng)基其發(fā)酵液對(duì)碳酸酐酶的抑制率可達(dá)91.43%,顯著高于未優(yōu)化的培養(yǎng)基(69.81%)。

【英文摘要】 In this study,the nutritional needs of Ganoderma applanatum to produce high active carbonic anhydrase inhibitors was investigated.Firstly,optimal carbon and nitrogen sources,and natural components were selected by single factor experi-ments according to carbonic anhydrase inhibition ratio of the fermentation broth.Then,inter-action of these factors was observed by orthogonal testsstyle.Finally,the inhibitory ratio based on basal medium and optimized medium was compared.The opti-mized medium contained: fructose 20 g.L-1,NaNO3 2 g.L-1,potato 175 g.L-1,(NH4)2SO4 1.3 g.L-1,In the medium,the highest inhibition ratio(91.43%) was achieved which was significantly higher than basal medium(69.81%).

【中文關(guān)鍵詞】 樹舌; 碳酸酐酶抑制劑; 培養(yǎng)基優(yōu)化
【英文關(guān)鍵詞】 Ganoderma applanatum; Carbonic anhydrase inhibitors; Medium optimization
【基金】國(guó)家973基金項(xiàng)目(40721002);; 江蘇安慧生物科技有限公司資助項(xiàng)目(項(xiàng)目編號(hào)2006278);; 江蘇大學(xué)高級(jí)人才專項(xiàng)基金資助(項(xiàng)目編號(hào)1283000268)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2009年06期 【DOI】CNKI:SUN:ZSYJ.0.2009-06-016