田文瑩; 邱芳萍; 劉洪霞; 王志兵
長(zhǎng)春工業(yè)大學(xué)化學(xué)與生命科學(xué)學(xué)院

【中文摘要】 肉湯常量稀釋法確定糞鬼傘粗提物對(duì)金黃色葡萄球菌、肺炎鏈球菌、埃希氏大腸桿菌、銅綠假單胞菌、白色念珠菌5種受試菌進(jìn)行體外抑菌試驗(yàn),測(cè)定最小抑菌濃度(MIC),并選取EAC、HepA和S180腫瘤細(xì)胞進(jìn)行體外抑瘤實(shí)驗(yàn)。結(jié)果表明,除對(duì)白色念珠菌沒(méi)有抑制作用外,糞鬼傘粗提物對(duì)受試細(xì)菌均有不同程度的抑菌效果,其中對(duì)金黃色葡萄球菌、肺炎鏈球菌、埃希氏大腸桿菌、銅綠假單胞菌的MIC分別為32mg·mL-1、16mg·mL-1、16mg·mL-1、32mg·mL-1。糞鬼傘粗提物對(duì)EAC、HepA和S180腫瘤細(xì)胞均有明顯的抑制作用,抑制率均在50%以上。

【英文摘要】 Aqueous extracts, isolated from the mycelium of liquid-cultured Coprinus sterqulinus Fr., were selected to investigate the antibacterial activity against 5 species of pathogenic and non-pathogenic microorganisms, such as Staphylococcus aureus, Ktebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The antibacterial activity of Coprinus sterqulinus Fr. extracts was measured in terms of the minimal inhibitory concentration (MIC) . The MIC of aqueous extracts against S. aureus, K. pneumoniae, E. coli, P. aeruginosa were 32 mg·mL-1, 16 mg·mL-1, 16 mg·mL-1, 32 mg·mL-1. However it did not exhibit antibacterial activity against C. albicans. Aqueous extracts of Coprinus sterqulinus Fr. showed better toxic effect on EAC, HepA and S180 cells, with the suppression ratio being higher than 50% for all these cell lines.

【中文關(guān)鍵詞】 抑菌活性; 糞鬼傘提取物; MIC; 體外抑瘤
【英文關(guān)鍵詞】 Antimicrobial activity; Coprinus sterqulinus Fr. extracts; MIC; Cellular toxicity
【基金】吉林省教育廳科技計(jì)劃項(xiàng)目,編號(hào):200660

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2009年05期 【DOI】CNKI:SUN:ZSYJ.0.2009-05-018