黃勇; 張金亭; 李金花; 趙麗茹; 于文斌; 代玉山; 孫麗麗
吉林省生物研究所; 一汽總醫(yī)院; 長春迪瑞藥業(yè)有限公司

【中文摘要】 從樹舌發(fā)酵菌絲體中提取水溶性粗多糖(GP),再從粗多糖中分離得到均一多糖并研究其單糖組成。采用DEAE cellulose及sephadex G200柱色譜法進一步純化得GPⅠ,并利用柱色譜、醋酸纖維素薄膜電泳等方法檢驗純度,GC和PC法測其單糖組成。結果表明,GPⅠ為均一中性雜多糖,相對分子質量為2000,單糖組成為Xyl、Man、Gal、Glc,摩爾比依次為0.027∶1.0∶5.88∶3.0,GPⅠ首次從樹舌菌絲體中分離獲得。

【英文摘要】 Homogeneous polysaccharide GPⅠ was isolated from GP which is a water-solube crude polysaccharides from hy- phostroma of Ganoderma applanatum and the composition of GPⅠ was studied. GPⅠwas purified by chromatography on DEAE cellulose column and sephadex G200 column. The homogencity of GPⅠ was proved by column chromatography and film elec-trophoresis of acetyl cellulose. The composition were studied by GC and PC. The results showed that GPⅠ was homogeneous, and its molecular weight was about 2 000. GPⅠ were composed of Xyl, Man, Gal, Glc with the molar ratio of 0.027∶1.0∶5.88∶ 3.0. GPⅠwas a neutral hetersaccharide which was obtained for the first time from the hyphostroma of Ganoderma applanatum.

【中文關鍵詞】 樹舌; 菌絲體; 多糖; 單糖組成
【英文關鍵詞】 Ganoderma applanatumn; Hyphostroma; Polysaccharides; Monosaccharide constitution
【基金】吉林省科技發(fā)展計劃項目(916028)

【文獻出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:ZSYJ.0.2009-03-019