吳映明; 陳愛葵; 彭錦紅; 梁美玲
廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系; 廣東教育學(xué)院生物系 廣州510303; 廣州510303; 廣州510303; 廣州510303

【中文摘要】 利用苯酚-硫酸法對(duì)破壁處理后的靈芝孢子粉多糖含量進(jìn)行測(cè)定,結(jié)果表明采用超低溫凍融處理和超聲波處理進(jìn)行靈芝孢子粉破壁均具有效果好、成本低及操作簡便等特點(diǎn);增加凍結(jié)-解凍循環(huán)次數(shù)在一定程度上能提高多糖含量,循環(huán)次數(shù)以5次～7次為宜;將超聲波處理法和超低溫凍融法很好地結(jié)合起來,其多糖釋放更高,效果更佳,此方法更具實(shí)用價(jià)值。

【英文摘要】 The measurement of polysaccharides in sporoderm-broken spore was analysed by the method of phenol-sulfuric acid. The result shows that it is an effective, economic and facile way to break the cellular wall with thawing and freezing of ultra-low temperture or ultrasonic. And the circulating times of thawing and freezing can increase the contents of polysaccharides in a certain degree and the sug-gested number is 5～7. The method of combining ultrasonic with thawing and freezing of ultra-low temperture commendably can release the polysaccharides of its contents higher and be of more practical value.

【中文關(guān)鍵詞】 靈芝孢子粉; 靈芝多糖; 超低溫凍融; 超聲波
【英文關(guān)鍵詞】 Ganoderma lucidum spore powder; Polysaccharides in Ganoderma lucidum; Thawing and freezing of ultra-low temperture; Ultrasonic

【文獻(xiàn)出處】 中國食用菌,Edible Fungi of China,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:ZSYJ.0.2008-03-016