沙濤; 丁驊孫
云南大學生物學系!云南昆明650091; 云南大學生物學系!云南昆明650091

【中文摘要】 本文應用改良的濱田氏松茸菌培養(yǎng)法對珍稀瀕危的食用菌松茸進行了菌絲體的組織分離及孢子分離 ,獲得了松茸組織分離純培養(yǎng)菌絲體。比較了幾種不同成分的培養(yǎng)基的分離效果 ,并對所獲菌絲體的形態(tài)特征進行了初步研究 ,經 2 6代移種馴化 ,其中 2支松茸組織分離菌絲體性狀穩(wěn)定 ,在改良的PDA培養(yǎng)基上生長良好

【英文摘要】 Isolation of Tricholoma matsutake was performed on several media from the organization and spore by Hamada's method.Filament products were obtained from the organization but no filament was observed due to the failure of germination of spore of T.matsutake.The features of these filaments were characterized. After transferring 26 generation,2 of them grow well on the modified potato dextrose agar medium.

【中文關鍵詞】 松茸; 組織分離; 孢子分離; 純培養(yǎng)菌絲體
【英文關鍵詞】 Tricholoma matsutake; Isolation; Filament

【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,2000年05期 【DOI】CNKI:SUN:ZSYJ.0.2000-05-003