尚德靜; 王關(guān)林; 方宏筠
遼寧師范大學(xué)食品生化研究所; 遼寧大連生物工程研究所!遼寧大連116029; 遼寧大連生物工程研究所!遼寧大連116029

【中文摘要】 本文探討了靈芝 (Ganodermalucidum)菌絲原生質(zhì)體的制備及再生的條件。實(shí)驗(yàn)結(jié)果表明 :采用MYG為靈芝菌絲培養(yǎng)基 ,2 %真菌溶壁酶 (Lywallzyme)作脫壁酶 ,0 6mol/L甘露醇作滲透壓穩(wěn)定劑 ;對(duì)培養(yǎng) 6d的靈芝菌絲在 30℃下進(jìn)行酶解 ,酶解時(shí)間 4- 6h ,可產(chǎn)生約 3 0× 1 0 6個(gè) /mL (0 1g菌絲 )原生質(zhì)體 ,并在添加靈芝子實(shí)體浸出汁的雙層培養(yǎng)基上實(shí)現(xiàn)了靈芝菌絲原生質(zhì)體的再生。

【英文摘要】 The conditions of protoplast formation and regeneration from the mycelia of Ganoderma lucidum were studied.Experimental results indicated that 3 0×10 6/mL protoplast of the mycelia of Ganoderma lucidum was obtained under optimal conditions,which are described as follows:Using 0 6mol/L mannitol as osmotic stabilizer;2% Lywallzyme as the enzyme digesting the cell wall;Using 6 days old MYG liquid cultures of the mycelia Ganoderma lucidum;at 30℃,enzyme digesting 4～6 hours.When incubated on two layer plates of agar,the protoplasts regenerated and formed new mycelia of Ganoderma lucidum.

【中文關(guān)鍵詞】 靈芝菌絲; 原生質(zhì)體
【英文關(guān)鍵詞】 Mycelia of Ganoderma lucidum; Protoplasts

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,2000年02期 【DOI】CNKI:SUN:ZSYJ.0.2000-02-000