劉標(biāo); 曹幼琴; 付庭治
南京大學(xué)生物科學(xué)與技術(shù)系

【中文摘要】 新分離到一株能產(chǎn)生較高水平菊粉酶的采絨革蓋菌（ＣｏｒｉｏｌｕｓｖｅｒｓｉｃｏｌｏｒＺＱＷ－９６）。通過對培養(yǎng)基組成及培養(yǎng)條件的優(yōu)化研究發(fā)現(xiàn)，用菊芋粉抽提液代替菊粉，氮源為玉米漿，添加０．０４％的吐溫—８０的發(fā)酵培養(yǎng)基，在ｐＨ５．８、２４℃、轉(zhuǎn)速為１５０ｒ／ｍｉｎ，裝液量為７５ｍｌ／５００ｍｌ三角瓶條件下發(fā)酵６天后，胞外菊粉酶活性達(dá)２．２４ｕ／ｍｌ，比原來提高了１．３倍。關(guān)于該菌產(chǎn)菊粉酶的研究，國內(nèi)外均未見報道

【英文摘要】 Coriolus versicolor strain ZQW-96 with higher inulinase activity was isolated. After studying of the optimal medium composition and culture conditions, we found that the strain's extracellular inulinase activity reached to 2 24u/ml with the medium whose carbon source was the extract of the tuber powder of Helianthus tuberosus instead of inulin, and nitrogen source was corn steep liquor, adding 0 04% Tween-80, and with the culture conditions was pH5 8, 24℃ 150r/min shaking, and six days' fermentation. This enzyme activity was 2 3 times higher than the initial. The report about the strain producing inulinase has never been found.

【中文關(guān)鍵詞】 采絨革蓋菌; 菊粉酶; 菊芋粉抽提液
【英文關(guān)鍵詞】 Coriolus versicolor ZQW-96; Inulinase; Extract of the tuber powder of Helianthus tuberosus

【文獻(xiàn)出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1997年03期 【DOI】CNKI:SUN:ZSYJ.0.1997-03-018