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    硒在金針菇菌體內(nèi)的生物轉(zhuǎn)化


    【發(fā)布日期】:2010-05-12

    胡文軍; 傅庭治; 曹幼琴
    南京大學生物科學與技術系

    【中文摘要】 本研究報導了液體深層培養(yǎng)金針菇(FlammulinavelutipesNJ9601)的富硒能力和生物轉(zhuǎn)化。在添加5~125(×10-6)硒的范圍內(nèi),菌絲體富硒的范圍為8.2~90.2(×10-6);添加20(×10-6)時,菌絲體吸收率最高,為對照的184%,此時菌體生長、可溶性蛋白質(zhì)、氨基酸、多糖等含量均高于對照組。無機硒經(jīng)菌體吸收、代謝、轉(zhuǎn)化,其有機化程度在75%以上,分布在硒蛋白(占64.1%),硒多糖(占11.2%)。用反相高效液相色譜法定量分析硒蛋氨酸含量。用富硒金針菇粉和剩余培養(yǎng)液分別飼養(yǎng)蚯蚓,其產(chǎn)量可提高30%以上。從飼養(yǎng)的蚯蚓提取分離獲得的蚓激酶產(chǎn)率提高20%。開辟了富硒金針菇綜合利用的新途徑

    【英文摘要】 In this paper, we report the ability of Flammulina uelutipes NJ9601, which cultivated in deep-liquid culture, to gather selenium and its biotransformation. When the selenium added to the culture is 5~125 (×10 -6 ), selenium contained in the hyphostroma is 8 2~90 2(×10 -6 ). When the selenium added to the culture is 20(×10 -6 ), the hyphostroma have the most selemium, which is 184% times more than that in the control. The dissoluble proteins, amino acids, polysaccharides in the hyphostroma wrer all more than those in the control groups. About 75% of nonorganic selenium that absorbed into the hyphostroma were transformed into organic selenium, which can be found in Se-proteins (64.1%), Se-polysaccharides(11.2%) and so on. The contents of Se-Met were quantitatively measured by reverse-high performance liquid chromotography. Applying the powders of Flammulina velutipes rich in selenium and the second-hand liquid cultures to earthworms, we found that the yield of earthworms could increase b...更多y 30% or more. And the yield of the fibrinolytic enzymes extracted from earthworms can increase by 20% or more. In this way, we open a new, comprehensive way to utilize Flammulina velutipes rich in selenium.  還原

    【中文關鍵詞】 金針菇; 硒蛋氨酸; 富硒
    【英文關鍵詞】 Flammulina velutipes; Se-Met; Se-concentration

    【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1997年03期 【DOI】CNKI:SUN:ZSYJ.0.1997-03-019

     
     
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