李英波; 羅信昌; 李濱; 郭良棟; 文華安
華中農(nóng)業(yè)大學(xué)應(yīng)用真菌研究所; 中國(guó)科學(xué)院微生物研究所

【中文摘要】 香菇（Ｌｅｎｔｉｎｕｓｅｄｏｄｅｓ）菌株的ＤＮＡ片段通過克隆轉(zhuǎn)移到質(zhì)粒載體ＢｌｕｅｓｃｒｉｐｔＭ１３—（ｐＢＳ）以獲得部分基因文庫(kù)。文庫(kù)中三個(gè)隨機(jī)克隆和一個(gè)密環(huán)菌中ｒＤＮＡ克隆被用作限制性片段長(zhǎng)度多態(tài)性探針。其中三個(gè)探針可檢測(cè)到多態(tài)性，揭示了３２個(gè)野生和栽培香菇菌林的遺傳分離。菌林間的相似性用平均鏈鎖聚類方式（ＵＰＧＭＡ）聚類，相似性超過６０％的分為一組。２３個(gè)菌株被分成４組。９個(gè)菌林與其它菌株相似性均低于６０％而獨(dú)立存在。從我們的結(jié)果看出，香菇的遺傳分離普遍存在于菌株間而與地理位置沒有相關(guān)性。ＲＦＬＰ具有研究香菇種內(nèi)遺傳分離的潛力，并可用作遺傳分析的遺傳標(biāo)記，因此能夠?yàn)橛N工作和遺傳分析提供科學(xué)依據(jù)。

【英文摘要】 EcoR Ⅰ restriction fragment of genemic DNA from the cultivated mushroom,Lentinus edodes,was cloned into the plasmid vector Bluescript MI 3 (pBS). Three randomclones from the genemic library and one rDNA clone of Armillaria melles were used to probefor restriction fragment length polymorphisms(RFLP).Three of the four probes detectedpolymorphisms and were used to demonstrate genetic divergence and detected polymorphismsin 32 wild and cultivated strains of Lentinus mushroom,The similarities between strains werecalculated using average linkage clustering (UPGMA),Based on 60 percent of similarity,23 strains were separated into four groups and anotlier 9 strains were not clustered intoany group due to their less similarity,From our resultS,the genetic divergence occurs amoungstrains but do not have a connection with geographic position,These results suggest thatRFLP have potentiaI to study interspecific genetic divergence in L.edodes and that RFLPcould be served as genetic markers for formal...更多 genetic analysis and thereby provide thefundation for breeding programmes.  還原

【中文關(guān)鍵詞】 香菇; RFLP; 遺傳分離; 遺傳標(biāo)記
【英文關(guān)鍵詞】 Lentinus edodes; RFLP; genetic divergence; genetic markers
【基金】國(guó)家自然科學(xué)基金

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1995年02期 【DOI】CNKI:SUN:ZSYJ.0.1995-02-004