王賢樵; 王澤生
福建省輕工業(yè)研究所; 福建省輕工業(yè)研究所 福州; 福州

【中文摘要】 采用聚丙烯酰胺凝膠垂直板狀電泳和瓊脂糖凝膠板狀電泳,研究不同生長(zhǎng)發(fā)育期,不同組織、不同培養(yǎng)基或栽培條件,對(duì)雙孢蘑菇8種同工酶和可溶性蛋白電泳表型的影響,發(fā)現(xiàn)酯酶、多酚氧化酶、細(xì)胞色素氧化酶、過(guò)氧化物酶、乙醇脫氫酶和可溶性蛋白的主要活性區(qū)帶存在與否,在營(yíng)養(yǎng)菌絲生長(zhǎng)階段或子實(shí)體發(fā)育階段的相同組織中不受上述各因素的影響,并發(fā)現(xiàn)不同類型菌株的電泳表型具有不同的活性區(qū)帶。把這類活性區(qū)帶命名為標(biāo)記區(qū)帶。把這類活性區(qū)帶命名為標(biāo)記區(qū)帶。以標(biāo)記區(qū)帶對(duì)同工酶表型進(jìn)行分類,發(fā)現(xiàn)酯酶同工酶是雙孢蘑菇生化鑒定或遺傳分析的最適標(biāo)記之一。

【英文摘要】 The effect of the different growing stages, different tissue and different mediums or cultivated environments on the electrophoretic patterns of eight isozymes and soluble protein from the different strains of Agaricus bisporus were investigated by the polyacrylamide slab gel electrophoresis (PAGE) and agarose gel electrophoresis (AGE). It was found that the appearance of main active bands of Esterase, Polyphenol oxidase, Cytoehrome oxidase, Peroxidase, Alcohol dehydrogenase and soluble protein from the vegetative hyphae or the same tissue of the fruit body were not influenced by the factors mentioned above and found there were different mein active bands in some of the different strains. The main active bands were designed as marker bands. When the isozymic patterns were classed according to the marker bands, it was found that Esterase isozyme is one of the most suitable genetic marker for Agaricus bisporus.

【中文關(guān)鍵詞】 雙孢蘑菇; 同工酶; 凝膠電泳; 標(biāo)記區(qū)帶; 遺傳標(biāo)記
【英文關(guān)鍵詞】 Agaricus bisporus; Isozyme; Gel electrophoresis; Marker bands; Genetic marker

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,1989年06期 【DOI】CNKI:SUN:ZSYJ.0.1989-06-002