【作者】應盛華 馮明光

【機構】浙江大學（華家池校區(qū)）生物科學系

【摘要】Thirty isolates of the entomophthotalean fungus, Conidiobolus coronatus, derived fromnaturally infected rice planthoppers were investigated for the achvihes of their alkaline protease.Culturing in sabouraud broth for 48h at 26℃, the isolates yield a dry mycelium biomass of 29.2mg / ml with a maximum of 32mg / ml. The activity of the alkaline protease in supemabot variedgreahy among the isolates, ranging 232-1410u / ml. The specific acihvity was high and venous:11000u / mg for 2 of the isolates tested, 8000-10000u / mg for 4 isolates, 5000-8000u / mg for 9isolates, and 2000-5000u / mg for 14 isolates. It is suggested that the alkaline protease is a highlyeffective achvator for vacuous prachcal pUIPOses. 

【基金】國家杰出青年科學基金!39525004；浙江省教委科學基金；

【關鍵詞】冠狀耳霉; 堿性蛋白酶; 昆蟲病原真菌;