反相高效液相色譜法測(cè)定瓦尼木層孔菌中柚皮素的含量
Determination of Naringenin Levels in Phellinus vaninii byReversed-phase High-performance Liquid Chromatography

【作者】 夏國華； 戈延茹； 戚雪勇； 鄭召磊； 任萌；

【Author】 XIA Guohua,GE Yanru,QI Xueyong,ZHENG Zhaolei,REN Meng(College of Pharmacy,Jiangsu University,Zhenjiang,Jiangsu 212013,China)

【機(jī)構(gòu)】 江蘇大學(xué)藥學(xué)院；

【摘要】 采用反相高效液相色譜法(RP-HPLC)測(cè)定瓦尼木層孔菌(Phellinus vaninii)中柚皮素含量。瓦尼木層孔菌醇提物水解后采用RP-HPLC對(duì)柚皮素的含量進(jìn)行測(cè)定,色譜條件:Hypersil ODS(4.6mm×100mm,3μm)色譜柱,流動(dòng)相為1%冰醋酸溶液-甲醇(62∶38),柱溫為40℃,流速為0.6mL/min,檢測(cè)波長(zhǎng)為288nm。標(biāo)準(zhǔn)曲線為A=4.18×106 X+40109(r=0.9998,n=5),柚皮素進(jìn)樣量在0.0202～2.020μg間線性關(guān)系良好,相對(duì)標(biāo)準(zhǔn)偏差(RSD)為1.56%(n=5),回收率為97.8%,重現(xiàn)性實(shí)驗(yàn)RSD為1.76%。該法適于瓦尼木層孔菌醇提物中柚皮素的含量測(cè)定。

【Abstract】 A method for determining naringenin levels in Phellinus vaninii fruit bodies by reversed-phase highperformance liquid chromatography(RP-HPLC)is described.Ethanolic(70v/v)extracts of P.vaninii fruit bodies were treated with 2% H2SO4at 100℃for 30mins.After adjusting the pH to 7.0with 40% NaOH,the solution was extracted with ethyl acetate and the extracts were freeze-dried.Samples(400 mg)of the freeze-dried material were dissolved in 25mL methanol,aliquots(20μL)of the solution were applied to a Hyp...

【關(guān)鍵詞】 反相高效液相色譜法； 瓦尼木層孔菌； 柚皮素；
【Key words】 RP-HPLC； Phellinus vaninii； naringenin；
 
【基金】 江蘇大學(xué)高級(jí)人才引進(jìn)專項(xiàng)資助項(xiàng)目(編號(hào):1283000138)的部分研究?jī)?nèi)容

【分類號(hào)】S567.39