兩種粵產(chǎn)粉孢牛肝菌抗氧化活性
Antioxidant Activity of Aqueous and Aqueous-Ethanolic Extracts of Two Tylopilus Species Endemic in Guangdong Province,China
【作者】 張明; 閆文娟; 李泰輝; 宋斌; 圖力古爾;
【Author】 ZHANG Ming1,2,YAN Wenjuan2,LI Taihui2,SONG Bin2,BAU Tolgor1 1Jilin Agriculture University,Changchun,Jilin 130118,China;2Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,State Key Laboratory of Applied Microbiology(MinistryGuangdong Province Joint Breeding Base),South China,Guangdong Open Laboratory of Applied Technology,Guangdong Institute of Microbiology,Guangzhou,Guangdong 510070,China
【機(jī)構(gòu)】 吉林農(nóng)業(yè)大學(xué); 廣東省微生物研究所,廣東省菌種保藏與應(yīng)用重點實驗室,廣東省華南應(yīng)用微生物重點實驗室-省部共建國家重點實驗室培育基地,廣東省微生物應(yīng)用新技術(shù)公共實驗室;
【摘要】 研究兩種產(chǎn)自廣東的粉孢牛肝菌(Tylopilus)——類鉛紫粉孢牛肝菌(T.plumbeoviolaceoides)和花蓋粉孢牛肝菌(GDGM27210)的子實體水提液和75%乙醇提取液的體外抗氧化活性。結(jié)果表明,在實驗濃度范圍內(nèi),兩種牛肝菌的子實體水提液和75%乙醇提液有不同程度的抑制亞油酸自氧化和清除自由基DPPH.、羥自由基.OH的作用。其中水提液對抑制亞油酸自氧化作用效果較好,最大抑制率分別可達(dá)95.74%和92.79%;而75%乙醇提取液對清除DPPH.效果較好,最大清除率分別可達(dá)86.68%和94.03%。
【Abstract】 Aqueous and 75% ethanolic extracts of fruit bodies of two Tylopilus species endemic in Guangdong Province were evaluated for in vitro antioxidant activity using free-radical scavenging-and lipid peroxidation-based assays.Extracts of Tylopilus plumbeoviolaceoides and Tylopilus sp.GDGM27210 inhibited autoxidation of linoleic acid and exhibited DPPH·and ·OH scavenging activity.Aqueous extracts of T.plumbeoviolaceoides and Tylopilus sp.GDGM27210 inhibited linoleic acid oxidation by approximately 45%...
【關(guān)鍵詞】 牛肝菌; 抗氧化活性; 自由基;
【Key words】 Tylopilus; antioxidant activity; free radical scavenging activity;
【基金】 國家基金項目(編號:30970023);廣東省科學(xué)院人才基金[編號:粵科優(yōu)(2008)01]、廣東省科學(xué)院野外臺站項目(編號:sytz201008)的部分研究內(nèi)容
【分類號】R371