猴頭菌提取物抗氧化活性研究
Antioxidant Activity of Hericium erinaceus Fruit Body Extracts
【作者】 潘偉; 劉瑞娜; 章爐軍; 俞康瑩; 尚曉冬; 趙國屏; 譚琦;
【Author】 PAN Wei1,2,LIU Ruina1,2,ZHANG Lujun1,YU Kangying1, SHANG Xiaodong1,ZHAO Guoping3,TAN Qi1 1Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences;Key Laboratory of Edible Fungi Resources and Utilization(South),Ministry of Agriculture,P.R.China;National Engineering Research Center of Edible Fungi;National R&D Center for Edible Fungi Processing;Key Laboratory of Agricultural Genetics and Breeding of Shanghai,Shanghai 201403,China;2College of Life Sciences,Nanjing Agricultural University, Nanjing,Jiangsu 210095,China;3Key Laboratory of Synthetic Biology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China
【機(jī)構(gòu)】 上海市農(nóng)業(yè)科學(xué)院食用菌研究所,農(nóng)業(yè)部南方食用菌資源利用重點實驗室,國家食用菌工程技術(shù)研究中心,國家食用菌加工技術(shù)研發(fā)分中心,上海市農(nóng)業(yè)遺傳育種重點開放實驗室; 南京農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院; 中國科學(xué)院上海生命科學(xué)研究院植物生理生態(tài)研究所;
【摘要】 分別采用還原力測定法、Fenton法、2,2-二苯基-1-苦肼基(DPPH)分析法和改良鄰苯三酚自氧化法,對猴頭菌子實體水提物和醇提物的總還原力,清除.OH、DPPH.和O2-.自由基的能力進(jìn)行測定。結(jié)果表明:醇提物還原力較強(qiáng),且還原力大小與濃度成正比;猴頭菌水提物和醇提物均有清除.OH、DPPH.和O2-.自由基的能力,且水提物的效果比醇提物好;水提物和醇提物對.OH、DPPH.和O2-.的清除能力依次為DPPH.、.OH和O2-.,并且在一定濃度范圍內(nèi),清除率與濃度成正比。
【Abstract】 The antioxidant activities of aqueous and 95%(v/v) ethanolic fractions of Hericium erinaceus fruit bodies,prepared by extraction at 60 ℃ for 20 mins,were evaluated by measuring both the total reducing power,and hydroxyl,DPPH and superoxide anion radical scavenging capability.Ethanolic extracts exhibited a higher total reducing power,which was concentration dependent,whereas aqueous extracts were more effective at scavenging hydroxyl,DPPH and superoxide anion radicals.
【關(guān)鍵詞】 猴頭菌; 清除自由基; 抗氧化活性;
【Key words】 Hericium erinaceus; free radical scavenging activity; antioxidant activity;
【基金】 上海市科技興農(nóng)重點攻關(guān)項目[編號:滬農(nóng)科攻字(2009)第2-2號];國家重點基礎(chǔ)研究發(fā)展計劃-973計劃(編號:2012CB721102);國家自然科學(xué)基金(編號:30830002);香港中文大學(xué)李嘉誠健康科學(xué)研究所基金(編號:7103506)的部分研究內(nèi)容
【分類號】R285