銀絲草菇菌絲體液體培養(yǎng)基篩選
Selection of Liquid Culture Media on Mycelium of Volvariella bombycina
【作者】 荊蓉; 郭成金;
【Author】 JING Rong,GUO Cheng-jin(College of Life Science,Tianjin Normal University,Tianjin 300387)
【機(jī)構(gòu)】 天津師范大學(xué)生命科學(xué)學(xué)院;
【摘要】 以銀絲草菇菌絲體生物量為主要指標(biāo),采用正交設(shè)計(jì)方法對(duì)其菌絲體液體培養(yǎng)基進(jìn)行了篩選,結(jié)果表明銀絲草菇菌絲體生長的最適培養(yǎng)基配方為:葡萄糖20 g.L-1、蔗糖10 g.L-1、酵母粉2.5 g.L-1、蛋白胨2 g.L-1、KH2PO4 2.5 g.L-1、MgSO4.7H2O 1.5 g.L-1、VB1 8 mg.L-1,pH自然。培養(yǎng)溫度28℃,周期168 h,其菌絲體生物量可達(dá)11.54 g.L-1。
【Abstract】 Using mycelium biomass of Volvariella bombycina as an major index,the liquid culture media of Volvariella bombycina mycelia was studied by means of orthogonal analysis.The results showed that the optimal medium for the mycelia was glucose 20 g·L-1,sucrose 10 g·L-1,yeast powder 2.5 g·L-1,peptone 2.5 g·L-1,KH2PO4 2.5 g·L-1,MgSO4·7H2O 1.5 g·L-1 and VB1 8 mg·L-1,natural pH.In the cultural condition of 28℃and 168 h,the mycelia biomass reached to 11.54 g·L-1.
【關(guān)鍵詞】 銀絲草菇; 液體培養(yǎng)基; 菌絲體; 生物量; 正交分析;
【Key words】 Volvariella bombycina; Liquid culture media; Mycelium; Biomass; Orthogonal analysis;
【分類號(hào)】S646.1