野生赭鱗蘑菇及其分離菌株的鑒定Identification of an Agaricus subrufescens Strain from Zhejiang Province,China
【作者】 宋小亞; 劉德云; 李陽(yáng); 顧新偉; 吳春玲;
【Author】 SONG Xiaoya1,LIU Deyun1,LI Yang1,GU Xinwei2,WU Chunling1(1Edible Fungi Institute,Lishui Agricultural Academy of Sciences,Lishui,Zhejiang 323000,China;2Lishui Agricultural Bureau,Lishui,Zhejiang 323000,China)
【機(jī)構(gòu)】 麗水市農(nóng)業(yè)科學(xué)研究院食用菌研究所; 麗水市農(nóng)業(yè)局;
【摘要】 依據(jù)形態(tài)學(xué)特征結(jié)合rDNA-ITS分析,對(duì)采集到的野生真菌進(jìn)行分類鑒定;采用RAPD分析生物學(xué)手段快速判斷其分離菌株的真實(shí)性,并通過(guò)出菇試驗(yàn)驗(yàn)證判斷結(jié)果。該野生真菌鑒定為赭鱗蘑菇(Agaricus subrufescens),RAPD分析和出菇試驗(yàn)結(jié)果表明分離菌株為該野生真菌的純培養(yǎng)物。
【Abstract】 Mushroom fruit bodies,collected from bamboo leaf piles near Bihu Town,Lishui City,Zhejiang Province,were identified as Agaricus subrufescens on the basis of sporophore and spore morphology,and ITS sequencing data.Pure cultures of fungal mycelium were obtained from fruit body tissue and confirmed as A.subrufescens on the basis of RAPD data and fruiting tests.
【關(guān)鍵詞】 野生真菌; 赭鱗蘑菇; 分離菌株; 鑒定;
【Key words】 Wild mushroom; Agaricus subrufescens; isolation of fungal mycelium; ITS sequencing;
【基金】 浙江省重大科技專項(xiàng)重點(diǎn)農(nóng)業(yè)項(xiàng)目(編號(hào):2009C12096);浙江省食用菌產(chǎn)業(yè)創(chuàng)新團(tuán)隊(duì)項(xiàng)目(編號(hào):2011R09029-13)的部分研究?jī)?nèi)容~~
【分類號(hào)】S646