猴頭菌不同生長發(fā)育期粗蛋白、粗多糖含量及水溶性粗多糖體外免疫活性
Protein,Polysaccharide and β-Glucan Levels in Hericium erinaceus Fruit Bodies Harvested at Different Development Stages and the Effect of Extracted Polysaccharide on NO Production by RAW264.7 Cells
【作者】 李巧珍; 劉朝貴; 于海龍; 蔣??; 劉艷芳; 張勁松; 唐慶九; 楊焱;
【Author】 LI Qiaozhen1,2,LIU Chaogui1,YU Hailong2,JIANG Jun2,LIU Yanfang2, ZHANG Jingsong2,TANG Qingjiu2,YANG Yan2 (1Key Laboratory of Vegetable Research of Chongqing,College of Horticulture and Landscape Architecture, Southwest University,Chongqing 400715,China;2National Engineering Research Center of Edible Fungi; Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China)
【機(jī)構(gòu)】 西南大學(xué)園藝園林學(xué)院重慶市蔬菜學(xué)重點(diǎn)實(shí)驗(yàn)室; 農(nóng)業(yè)部南方食用菌資源利用重點(diǎn)實(shí)驗(yàn)室國家食用菌工程技術(shù)研究中心上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室上海市農(nóng)業(yè)科學(xué)院食用菌研究所;
【摘要】 測(cè)定猴頭菌(Hericium erinaceus)華中猴頭菌株不同生長發(fā)育期子實(shí)體中的粗蛋白、粗多糖和水溶性粗多糖中β-葡聚糖含量,并研究不同生長發(fā)育期子實(shí)體粗多糖體外刺激巨噬細(xì)胞產(chǎn)生NO的活性變化規(guī)律。結(jié)果表明,供試菌株不同生長發(fā)育期子實(shí)體的粗蛋白、粗多糖和β-葡聚糖含量以及粗多糖體外刺激巨噬細(xì)胞產(chǎn)生NO的活性均存在顯著或極顯著差異,隨著生長發(fā)育的進(jìn)行,猴頭菌子實(shí)體中粗蛋白含量總體上呈下降的趨勢(shì)(小菌刺期比前一時(shí)期有所增加),而多糖含量則總體呈上升趨勢(shì)(小菌刺期比后一時(shí)期高),β-葡聚糖含量呈先快速上升后平穩(wěn)的趨勢(shì);分裂期粗多糖在濃度500μg/mL時(shí)對(duì)RAW264.7釋放NO的促進(jìn)作用較強(qiáng)。
【Abstract】 Fruit bodies of Hericium erinaceus,strain Huazhong,were harvested at seven different developmental stages,dried and pulverized to pass a mesh size of 0.9 mm.Determination of protein levels in the powdered material,and polysaccharide levels in crude aqueous extracts,revealed that,in general,the former decreased as mushroom development progressed whereas the latter increased.The concentration of β-glucan in extracted polysaccharide material was much higher in samples taken at the second developmen...
【關(guān)鍵詞】 猴頭菌; 生長發(fā)育期; 粗蛋白; 粗多糖; β-葡聚糖; NO釋放量;
【Key words】 Hericium erinaceus; fruit body developmental stage; protein; crude polysaccharide; β-glucan; NO production;
【基金】 上海市農(nóng)委重點(diǎn)攻關(guān)項(xiàng)目[編號(hào):滬農(nóng)委攻字(2008)第8-2號(hào)]的部分研究內(nèi)容
【分類號(hào)】S646.9